Hepatocellular Carcinoma (HCC) is one of the most frequent cancers in Egypt where there is high prevalence of infection with the Hepatitis C virus (HCV). Matrix metalloproteinases (MMPs) are multifunctional proteins that play an important role in cell development, differentiation, inflammation and angiogenesis. Polymorphisms in MMPs genes might be involved in development of HCV related HCC. The aim of this study was to explore the relationship of gene polymorphisms in MMP-1,3 & 9 with liver cirrhosis and HCC patients.
The study included 128 subjects enrolled from Menoufia University Hospital inpatients and outpatients clinics from the Department of Clinical Oncology & Nuclear Medicine and Department of Tropical Medicine in the period between October 2015 and August 2016. Patients were classified into three groups. Group I: 48 patients with HCC in addition to liver cirrhosis, including 26 males and 22 females with a mean age of 58.60±5.29; Group II: 50 patients with liver cirrhosis, including 26 males and 24 females with a mean age of 56.74±5.21; Group III: 30 healthy subjects as controls, including 15 males and 15 females with a mean age of 56.30 ±7.30. Diagnosis of HCC was performed using two imaging methods (abdominal US & triphasic CT). All subjects except controls were positive for serum HCV RNA. Liver function tests, AFP & CHILD score were assessed. Gene polymorphisms were analysed using PCR-RFLP.
HCC patients had higher mutant G2G2 (35.4%) and G2 allele (62.5%) of the MMP-1 gene than patients in both cirrhotic (P
Gene mutations in MMP-1,3, 9 may be involved in progression of liver cirrhosis and risk relationship for HCC development.
Clinical trial identification
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All authors have declared no conflicts of interest.