Nowadays, patients with Hodgkin’s lymphoma (HL) have a high cure rate. However, approximately 20% pts have relapse or progressive disease. The main histological feature in HL is presence by Hodgkin Reed-Sternberg (HRS) cells. HRS cells produce a diversity of cytokines and chemokines. There is still a limited information of prognostic role expression of cytokines in patients with HL. We assessed the impact on clinical outcome of the IDO, TGF- β and IL-13 expression in patients with HL.
79 patients (pts) with HL were included in this study (median age: 41, range: 18-65 years; males: 26, females: 53). Early stages of HL have 53.3% and 46.7% pts had advanced stages. Patients were treated with ABVD or BEACOPP (14/esc) and radiation therapy. CR/PR was achieved in 86.1% of patients. 13.9% of pts had relapse or disease progression during the therapy. mRNA expression levels of IDO, TGF-β, IL-13 were measured in fresh pre-treatment tumor tissue specimens from HL patients using real-time qPCR analysis.
25 pts (31.6%) had IDO positive expression (IDO+) and 54 (65.4%) pts was IDO negative (IDO-). Multivariate analysis showed that IDO+ expression correlated with worser EFS rate with HRs of 10.7 [95% confidence interval(CI) 0.4–0.6, p = 0.01], especially in 3-years EFS in IDO+ males comparing to IDO+ females (61% vs 71%, p
Our data showed that IDO, TGF-β and IL13 expressions have a role in predicting clinical outcome in pts with HL. Positive expression of IDO, TGF-β and high expression level of IL13 could be considered as a negative prognostic marker. Significantly worser outcome had pts with positive expression of two cytokines comparing with patients, who had only one positive marker.
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All authors have declared no conflicts of interest.