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Poster display session

5026 - Characterization of cancer stem cell and immune microenvironment interactions in non-small cell lung cancer (NSCLC).

Date

09 Sep 2017

Session

Poster display session

Presenters

Susana Torres

Citation

Annals of Oncology (2017) 28 (suppl_5): v453-v456. 10.1093/annonc/mdx381

Authors

S. Torres1, R. Sirera2, C. Aguilar1, S. Calabuig Fariñas3, M. Mosqueda1, A. Herreros-Pomares1, E. Escorihuela4, E. García del Olmo5, E. Jantus-Lewintre6, C. Camps Herrero7

Author affiliations

  • 1 Laboratorio De Oncología Molecular, Fundación para la Investigación, Hospital General Universitario de Valencia, 46020 - Valencia/ES
  • 2 Departamento De Biotecnología, Universitat Politècnica de València-CIBERONC, 46022 - Valencia/ES
  • 3 Laboratorio De Oncología Molecular, Fundación Para La Investigación, Hospital General Universitario De Valencia-ciberonc;, Departamento de Patología, Universitat de València., 46020 - Valencia/ES
  • 4 Laboratorio De Oncología Molecular, Fundación para la Investigación, Hospital General Universitario de Valencia-CIBERONC, 46020 - Valencia/ES
  • 5 Cirugía Torácica, Hospital General Universitario de Valencia, 46020 - Valencia/ES
  • 6 Laboratorio De Oncología Molecular, Fundación Para La Investigación, Hospital General Universitario De Valencia-ciberonc, Departamento de Biotecnología, Universidad Politécnica de Valencia, 46020 - Valencia/ES
  • 7 Laboratorio De Oncología Molecular, Fundación Para La Investigación, Hospital General Universitario De Valencia-ciberonc, Servicio de Oncología Médica, Hospital General Universitario de Valencia; Departamento de Medicina, Universidad de Valencia, 46018 - Valencia/ES
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Resources

Abstract 5026

Background

Lung cancer stem cells (CSCs) are a small subpopulation of cells with self-renewal, tumorigenic properties and the ability to grow forming tumourspheres in non-adherent conditions. CSCs in non-small cell lung cancer (NSCLC) are targets poorly recognized by the immune surveillance system given that they favour an immunosuppressive microenvironment. The aim of this work was to compare the release of cytokine between monolayer cells and tumourspheres.

Methods

The study was performed on medium supernatant of cells from two NSCLC tumour patients (patient 1 and patient 2) samples and ten cell lines (A549, H1650, H460, H23, H358, H2228, HCC827, PC9, H1993, and SW900) grown in monolayer and tumourspheres at 3 different densities (104, 5·105 and 105 cells/ml). We analysed four soluble factors with immunosuppressive (IL-4, IL-10), and immunoregulatory (IL-6, IL-8) capacity through sensitivity bead-based multiplex assay using the Millipore kit and the Luminex 100/200.

Results

All human tumour cell lines and primary cells secreted detectable levels of IL-6 and IL-8. In contrast, IL-10 and IL-4 levels were below detectable range (1750.122177.4>2273.3H35810.927.81774.8>2273.3H199334.42.2658.2479.3PC9144.45.4597.2321.9SW900121.75.41006225.2H460359.8227.81298.82201.3H233.72.8407.81290.8H1650995.9352.230.250.3Patient 117.40.6>2273.330.0Patient 2>1750.12>1750.12>2273.3>2273.3

Conclusions

Our preliminary results suggest that cells grown in adherence show increased levels of IL-6 compared to lung-tumourspheres. The next step is the expansion of the cohort to obtain significant results.

Supported by grants from FEDER and PI12-02838 and PI15-00753 from ISCIII.

Clinical trial identification

Legal entity responsible for the study

Fundación para la Investigación del Hospital General Universitario de Valencia

Funding

Supported by grants from FEDER and PI12-02838 and PI15-00753 from ISCIII.

Disclosure

All authors have declared no conflicts of interest.

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