The aim of our study was a comprehensive study of molecular-genetic, pathologic and clinical symptoms in patients with osteosarcoma to enhance the effectiveness of early diagnosis, treatment and prevention of osteosarcomas.
We studied the immunophenotypic characteristics of tumor cells in 212 patients with osteosarcoma. Results of antibody reactions to Ki-67, bcl-2, p53 (mutant gene) localized in the nuclei and the mitochondrial matrix expressed in % based on the number of stained cells per 100 examined.
Our results revealed that the expression profile of p53 +, bcl-2-, Ki 67+ in 34.9% (74/212) patients with osteosarcoma is considered as poor prognosis, presented as early metastasis, progression of tumor growth and early relapses (15 months), advanced processes (III and IV stage), low grade pathomorposis (1 and 2), the relative duration of lifespan in patients (up to 3 years), it is associated with a low degree of differentiation (G3), an increase in tumor size up to 550 cm3 with chondroblastic type of osteosarcoma. These data should be considered when looking for and isolating the most promising groups for molecular genetic markers that are would be predictive valuable in the clinic for monitoring the treatment of patients with osteosarcoma.
Thus, it is obvious need for clinical medicine in the implementation and expansion of molecular testing for effective decision-making on the appointment of molecular targeted therapy of patients with osteosarcoma. These requires optimization the approach based on consideration of the specifics of the population, the objective conditions related to the presence of intratumoral characteristics. The data reveals a group of patients with osteosarcoma at high risk with unfavorable prognosis at the stage of examination and choose for these kind of patients effective therapy.
Clinical trial identification
Legal entity responsible for the study
National Cancer Research Center of Uzbekistan
All authors have declared no conflicts of interest.