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Poster display session

5073 - Analysis of liquid biopsies from metastatic colorectal carcinoma (mCRC) patients (pts) enrolled in the CAPRI GOIM clinical trial

Date

09 Sep 2017

Session

Poster display session

Presenters

Nicola Normanno

Citation

Annals of Oncology (2017) 28 (suppl_5): v158-v208. 10.1093/annonc/mdx393

Authors

N. Normanno1, R. Esposito Abate1, M. Lambiase1, L. Forgione1, C. Cardone2, A. Iannaccone1, A. Sacco1, A.M. Rachiglio1, E. Martinelli3, D. Rizzi4, S. Pisconti5, G. Cartenì6, R. Bordonaro7, T. Troiani3, F. Giuliani8, S. Leo9, S. Romito10, A. Rinaldi11, E. Maiello12, F. Ciardiello13

Author affiliations

  • 1 Cell Biology Unit, Istituto Nazionale Tumori – I.R.C.C.S - Fondazione Pascale, 80131 - Napoli/IT
  • 2 Oncologia Medica, Dipartimento Di Internistica Clinica E Sperimentale “f. Magrassi”,, Università degli Studi della Campania Luigi Vanvitelli, 80121 - Napoli/IT
  • 3 Oncologia Medica, Dipartimento Di Internistica Clinica E Sperimentale “f. Magrassi”,, Università degli Studi della Campania Luigi Vanvitelli, 80131 - Napoli/IT
  • 4 Trial Office, GOIM, Bari/IT
  • 5 Oncologia Medica, Ospedale S. Giuseppe Moscati, Statte/IT
  • 6 Medical Oncology, Azienda Ospedaliera di Rilievo Nazionale "Antonio Cardarelli"-AORN A. Cardarelli, 80131 - Napoli/IT
  • 7 Medical Oncology, Azienda Ospedaliera ARNAS Garibaldi, 95100 - Catania/IT
  • 8 Medical Oncology, Istituto Tumori Giovanni Paolo II, 70126 - Bari/IT
  • 9 Geriatric Oncology, Ospedale Vito Fazzi, 73100 - Lecce/IT
  • 10 Oncology, Ospedale Riuniti di Foggia, 71100 - Foggia/IT
  • 11 Oncologia, ASL Taranto, 74011 - Castellaneta/IT
  • 12 Medical Oncology, IRCCS Casa Sollievo della Sofferenza, 71013 - San Giovanni Rotondo/IT
  • 13 Dipartimento Medico-chirurgico Di Internistica Clinica E Sperimentale "f. Magrassi E A. Lanzara", Università degli Studi della Campania "Luigi Vanvitelli", 80131 - naples/IT
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Resources

Abstract 5073

Background

Liquid biopsy can represent an alternative to tissue biopsy for biomarker testing in cancer pts. In addition, liquid biopsy can be used to monitor the response to treatment and the molecular evolution of the disease.

Methods

In the CAPRI GOIM trial, KRAS exon 2 wild type (wt) mCRC pts received first line cetuximab plus FOLFIRI. Tumor samples were assessed by Next Generation Sequencing (NGS) with the Ion AmpliSeq™ Lung and Colon Cancer Panel (Thermofisher). Plasma samples at baseline (n = 96), at 3 weeks of treatment (n = 54), at 6 weeks (n = 14) and at progression of disease (n = 24) were collected from 96 patients and analyzed for exon 2, 3 and 4 KRAS and NRAS mutations using BEAMing Digital PCR (Sysmex Inostics).

Results

Analysis of basal plasma samples from the 96 pts included in this study showed a concordance of 79,2% with the tissue RAS status as defined by NGS. The 11 cases that were RAS mutant (mut) in tissue and wt in plasma had suboptimal plasma volume available for analysis (

Conclusions

These data suggest that liquid biopsy might better recapitulate the heterogeneity of mCRC and might be useful to monitor the response to therapy. Analysis are ongoing to evaluate the clinical significance of RAS mutations with low MAF.

Clinical trial identification

Eudract number: 2009-014041-81

Legal entity responsible for the study

Nicola Normanno

Funding

Merck Serono, Sysmex Inostics

Disclosure

N. Normanno: Participation to advisory boards and/or research funding from Amgen, AstraZeneca, Merck Serono, MSD, Qiagen, Roche, Sysmex. E. Martinelli, T. Troiani: Participation to advisory boards: Servier and Roche. E. Maiello: Participation to advisory boards: Merck Serono, Roche, Sanofi. F. Ciardiello: Participation to advisory boards and/or research funding from Amgen, Bayer, AstraZeneca, Merck Serono, Roche. All other authors have declared no conflicts of interest.

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