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Gynaecological cancers

5039 - An increased ratio of cytotoxic to suppressive T cells after neoadjuvant chemotherapy (NACT) is prognostic in advanced ovarian cancer

Date

09 Sep 2017

Session

Gynaecological cancers

Presenters

Aya Khairallah

Citation

Annals of Oncology (2017) 28 (suppl_5): v330-v354. 10.1093/annonc/mdx372

Authors

A. Khairallah1, A. Auguste2, A. Leary3, C. Genestie4, P. Pautier5, J. Michels3, P. Morice3, E. Bentivegna3, A. Maulard3, F. Drusch3, S. Mesnage5, S. Gouy5

Author affiliations

  • 1 Pathology, GUSTAVE ROUSSY, 94800 - Villejuif/FR
  • 2 Oncology, GUSTAVE ROUSSY, 94800 - Villejuif/FR
  • 3 Gynaecologic Oncology, Gustave Roussy Cancer Center, INSERM U981, and Groupe d'Investigateurs Nationaux pour l'Etude des Cancers Ovariens (GINECO), 94800 - Villejuif/FR
  • 4 Department Of Pathology, Institut Gustave Roussy, 94800 - Villejuif/FR
  • 5 Medical Oncology, Gustave Roussy Institut de Cancérologie, 94805 - Villejuif/FR
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Resources

Abstract 5039

Background

At diagnosis, tumor-infiltrating lymphocytes (TILs) are prognostic in epithelial ovarian cancer (EOC). We recently demonstrated that neoadjuvant chemotherapy (NACT) significantly increased stromal TILs and stromal TILs remained prognostically significant after NACT. Here, we investigated the impact of NACT on different immune subpopulations and their relationship with clinical outcome.

Methods

Tissue microarrays of EOC (145 pre-NACT, 139 post-NACT, including 83 matched samples) were analyzed for CD3+,CD8+ and FOXP3+ by immunohistochemistry. Stromal TILs scored as percentage of stromal area, intraepithelial TILs as number of TILs in contact with tumor cells/HPF. Differences were evaluated by Mann-Whitney or Wilcoxon-signed-rank for unpaired or paired analyses, respectively and by Log-rank for PFS and OS.

Results

NACT significantly increased stromal CD3 + (p = 0.005) and CD8 + (p = 0.009) and intraepithelial CD8 + (p = 0.02) infiltration in unmatched samples and remained significant among paired samples for stromal CD3+ and CD8 + (p = 0.03 and p = 0.009). Neither CD3+ nor CD8+ expression correlated with outcome at diagnosis or post-NACT, however reduced accumulation of FOXP3+ post-NACT (

Conclusions

NACT has a significant impact on the balance of cytotoxic versus suppressive T cells and a high ratio of CD8+/FOXP3+ post-NACT was most significantly associated with improved PFS and OS. Whether this could select patients for immune therapies in the post-operative setting should be investigated.

Clinical trial identification

none

Legal entity responsible for the study

Institut Gustave Roussy

Funding

INCA and MERUS

Disclosure

All authors have declared no conflicts of interest.

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