Oops, you're using an old version of your browser so some of the features on this page may not be displaying properly.

MINIMAL Requirements: Google Chrome 24+Mozilla Firefox 20+Internet Explorer 11Opera 15–18Apple Safari 7SeaMonkey 2.15-2.23

Poster display

720 - Validation of novel diagnostic kits using the semi-dry dot-blot method for detecting metastatic lymph nodes in breast cancer; distinguishing macrometastases and micrometastases


10 Oct 2016


Poster display


Ryota Otsubo


Annals of Oncology (2016) 27 (6): 401-406. 10.1093/annonc/mdw380


R. Otsubo1, H. Hirakawa2, M. Oikawa3, A. Tanaka1, M. Matsumoto1, H. Yano1, N. Kinoshita4, K. Abe4, J. Fukuoka4, T. Nagayasu1

Author affiliations

  • 1 Department Of Surgical Oncology, Nagaski University Hospital, 852-8501 - Nagasaki/JP
  • 2 Gynecology, Chiba Aiyuukai Memorial Hospital, Chiba/JP
  • 3 Breast Surgery, Oikawa Hospital, Fukuoka/JP
  • 4 Department Of Pathology, Nagaski University Hospital, 852-8501 - Nagasaki/JP


Abstract 720


The semi-dry dot-blot (SDB) method is a diagnostic procedure for detecting lymph node (LN) metastases. Metastases are confirmed by the presence of cytokeratin (CK) in lavage fluid of sectioned LNs that contain anti-pancytokeratin antibody, based on the theory that epithelial components such as CK are not found in normal LNs. We evaluated two novel SDB kits that use the newly developed anti-CK19 antibody for diagnosing LN metastases in breast cancer.


We obtained 139 LNs dissected from 79 breast cancer patients from July 2013 to April 2015 at Nagasaki University Hospital, including 32 dissected axillary LNs and 107 sentinel LNs, sliced at 2-mm intervals and washed with phosphate-buffered saline. The suspended cells in the lavage fluid of sliced LNs were centrifuged and lysed to extract protein. This extracted protein was used with a low-power and a high-power kit to diagnose LN metastasis. The washed LNs were blindly diagnosed by pathologists using hematoxylin and eosin (H&E) stain. Diagnoses based on the kit were compared with their H&E counterparts.


Of the 139 LNs, 55 were assessed as positive and 84 as negative by permanent pathological examination with H&E. Sensitivity, specificity, and accuracy of the low-power kit for detecting LN metastases was 80.7%, 100%, and 92.2%, respectively. In 10 false-negative cases, there were eight micrometastases, producing a sensitivity of 95.3% for detecting macrometastases. Sensitivity, specificity, and accuracy of the high-power kit for detecting LN metastases was 90.9%, 91.7%, and 92.1%, respectively. Combining the low- and high-power kit results, sensitivity, specificity and accuracy for distinguishing macrometastases from micrometastases was 95.3%, 95.8%, and 95.7%, respectively. Diagnosis was achieved in approximately 20 min using the kits, at a cost of less than 25 USD.


The kits in our study were accurate, quick, and cost-effective in diagnosing LN metastases without the loss of LN tissue. The kits' ability to distinguish macrometastases from micrometastases was excellent, which is important, clinically.

Clinical trial identification

Legal entity responsible for the study

Ryota Otsubo


Nagasaki University Hospital, Department of Surgical Oncology


All authors have declared no conflicts of interest.

This site uses cookies. Some of these cookies are essential, while others help us improve your experience by providing insights into how the site is being used.

For more detailed information on the cookies we use, please check our Privacy Policy.

Customise settings