Abstract 1783
Background
In primary tumors (P), infiltrating lymphocytes (TIL) and tertiary lymphoid structures (TLS) have been associated with better clinical outcomes, in HER2+ and triple negative breast cancer (BC). The temporal and spatial heterogeneity of TIL and TLS between P and their matched metastases (M) is currently unknown.
Methods
We analyzed formalin fixed paraffin embedded tissue samples from a retrospective cohort of BC patients who underwent adjuvant surgery for the P and metastasectomy or biopsy for their metachronous M at a distant site(s) (n = 51). Immunohistochemistry stained sections using anti-CD3 (T cells) and anti-CD20 (B cells) antibodies in a double stain were scored by two trained pathologists blinded to the clinical data. TIL were scored as the percent (%) of stroma plus tumor surface area infiltrated with CD3+ plus CD20+ cells. The number of TLS were normalized to the tumor area.
Results
The extent of TIL in P (range 1-35%) was significantly higher with respect to the corresponding M (p = 0.04). Interestingly, when the extent of TIL in P was ≥10% (=TIL+) there was a significant decrease in the %TIL detected in M (p
Conclusions
These preliminary data suggest that tumors can alter their immunogenicity during disease progression by their production of neoantigens and/or immunosuppressive factors, which potentially accounts for our observed differences between P and M. In metastatic disease, the extent of immune infiltration is globally lower than in early disease with almost no TLS found in M, signifying that the composition and organization of the immune infiltrate varies between disease stages. The extent of the immune infiltrate may also depend upon the organ site of relapse, further complicating the heterogeneity of both the tumor and the anti-tumor immune response.
Clinical trial identification
Legal entity responsible for the study
Universitè Libre de Bruxelles
Funding
Les Amis de l'Institut Jules Bordet
Disclosure
All authors have declared no conflicts of interest.