Survivin gene expression in the primary tumor and circulating tumor cells – a new biomarker of tumor progression of breast cancer

Date

10 Oct 2016

Session

Poster display

Presenters

Yauheni Shliakhtunou

Citation

Annals of Oncology (2016) 27 (6): 15-42. 10.1093/annonc/mdw363

Authors

Y. Shliakhtunou

Author affiliations

  • Oncology, Vitebsk State Medical University, 210023 - Vitebsk/BY
More

Resources

Background

Breast cancer is a leading cause of morbidity and mortality of the female population from malignant tumors. Distant metastases are the main cause of death of patients, a substrate for the development of which are circulating tumor cells (CTCs). However, the search for these cells alone is not sufficient to provide full information about the nature and course of tumor in a single patient. Determination of the expression of tumor-genes responsible for the different processes of tumor progression allows a more complete picture. Such genes include the gene survivin (BIRC5) family of inhibitors of apoptosis (IAP).

Methods

Using real-time PCR we investigated the expression of the survivin gene in 36 samples of primary invasive ductal carcinoma of the breast, 10 samples of benign tumors - fibroadenoma of the breast, as well as 36 samples of peripheral blood of patients with breast cancer at various stages of tumor and stage specific treatment, and 10 healthy people as controls.

Results

In primary breast carcinoma we determined a high expression of survivin gene in all 36 samples with the average value (M ± m) 1.58 ± 0.31 (min – 1.19; max – 4.41). The highest figures were found in tumors of medium and high grade (G II-III) with lymphovenous invasion (LVSI +). In 2 of 3 samples of benign tumor expression of survivin was not found, and one was 0.015. In CTCs, isolated from peripheral blood of breast cancer patients, all 36 samples as determined by the gene expression of survivin with an average value (M ± m) 1.10 ± 0.19 (min – 0.36; max – 3.79). The level of expression of the control samples did not exceed 0.003. It should be noted that the maximum volume of expression was obtained in samples of tumor patients with stage N +, and especially M1, on TNM classification. Any legitimate expression of survivin, depending on the size of the tumor had been received. In patients, receiving chemotherapy average expression of survivin gene was obswerved, but it never approached the indicators of control.

Conclusions

Determination of expression of the survivin gene in primary tumor and in CTCs may be one of the most promising markers of tumor progression and for monitoring of breast cancer therapy.

Clinical trial identification

Legal entity responsible for the study

Vitebsk State Order of Peoples' Friendship Medical University

Funding

Belarusian Republican Foundation for Fundamental Research

Disclosure

All authors have declared no conflicts of interest.

Resources from the same session

This site uses cookies. Some of these cookies are essential, while others help us improve your experience by providing insights into how the site is being used.

For more detailed information on the cookies we use, please check our Privacy Policy.

Customise settings