Oops, you're using an old version of your browser so some of the features on this page may not be displaying properly.

MINIMAL Requirements: Google Chrome 24+Mozilla Firefox 20+Internet Explorer 11Opera 15–18Apple Safari 7SeaMonkey 2.15-2.23

Poster Display

2391 - Prognostic value of the immune-related transcriptome in biliary tract cancers


08 Oct 2016


Poster Display


Michele Ghidini


Annals of Oncology (2016) 27 (6): 207-242. 10.1093/annonc/mdw371


M. Ghidini1, L. Cascione2, A. Lampis3, R. Pandolfo4, P. Carotenuto4, F. Trevisani3, J.C. Hahne3, D. Zito3, V. Guzzardo5, A. Zerbi6, G. Torzilli6, M. Roncalli7, L. Rimassa1, A. Santoro1, M. Fassan5, N. Valeri3, C. Braconi4

Author affiliations

  • 1 Medical Oncology, Istituto Clinico Humanitas, 20089 - Rozzano/IT
  • 2 Bioinformatics Core Unit, IOSI Istituto Oncologico Svizzera Italiana Ospedale Regionale Bellinzona e Valli, Bellinzona/CH
  • 3 Laboratory Of Gastrointestinal Cancer Biology And Genomics, Division Of Molecular Pathology, The Institute of Cancer Research ICR, SM2 5NG - Sutton/GB
  • 4 Cancer Therapeutics, The Institute of Cancer Research ICR, SM2 5NG - London/GB
  • 5 Internal Medicine, Azienda Ospedaliera di Padova Università, Padova/IT
  • 6 Surgical Oncology, Istituto Clinico Humanitas, 20089 - Rozzano/IT
  • 7 Pathology, Istituto Clinico Humanitas, 20089 - Rozzano/IT


Abstract 2391


Biliary Tract Cancers (BTC) are inflammatory cancers with derangement of cytokines and recruitment of immune cells. Here we investigate if a transcriptomic immune profile (IP) is activated in BTC and represents a feature of biological behaviour.


RNA was extracted by tumour tissues (TT) and matched adjacent tissues (AT). IP of 700+ immune-related transcripts was performed in TT and AT of 24 BTCs by nCounter assay. CD80 expression was assessed by immunohistochemistry (IHC). Cox regression analysis and Kaplan Meier methods were used to correlate with Relapse Free Survival (RFS) and derive a prognostic gene signature.


132 transcripts were aberrantly expressed in TT vs AT. Ingenuity Pathway Analysis showed that leucocyte migration was the top function annotation. De-regulation of PDZ Binding Kinase (PBK) in TT appeared to differentiate cases with worse prognosis (nCounter p:0.08; Taqman p:0.07). We derived a list of genes whose expression was associated with RFS. We observed that risk of recurrence was associated with a greater number of genes deregulated in AT than in TT. We shortlisted genes that maintained statistical significance at multivariate analysis (gene expression, tumour site, adjuvant chemotherapy (AC) and R0/1 resection). We observed correlation between high expression of cytotoxic T-lymphocyte antigen-4 (CTLA-4) in the AT and RFS (p:0.0004). Cases with low CTLA4 had reduced expression of CD80, while cases with high CTLA4 had increased CD80 expression. IHC expression of CD80 varied in TT and AT. No association was seen between AT CD80 expression and RFS. However, CD80 expression seemed to differentiate prognosis in patients who did not receive AC (p:0.01), suggesting that activation of this pathway may promote relapse and may be affected from adjuvant treatment. Number of CD8+ and CD4+ cells did not correlate with RFS. We also derived a immuno-gene signature that could significantly differentiate relapsed cases and was an independent prognostic factor (HR 29.43, p:0.001).


We showed that IP is deregulated in the AT of resected BTC and correlates with relapse suggesting that deregulation of immune infiltrate in the normal tissue creates a favourable soil for cancer cell growth.

Clinical trial identification

Legal entity responsible for the study



Institute of Cancer Research.


All authors have declared no conflicts of interest.

This site uses cookies. Some of these cookies are essential, while others help us improve your experience by providing insights into how the site is being used.

For more detailed information on the cookies we use, please check our Privacy Policy.

Customise settings