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Poster display

2839 - Prevalence, pattern, and impact of PD-L1 expression and HPV-status in head and neck squamous cell carcinoma


09 Oct 2016


Poster display


Teeranuch Chureemas


Annals of Oncology (2016) 27 (6): 328-350. 10.1093/annonc/mdw376


T. Chureemas1, N. Larbcharoensub2, J. Juengsamarn1, T. Layangkool3, C. Jiarpinitnun4, P. Chansriwong1, N. Trachu5, P. Pattaranutaporn4, N. Ngamphaiboon1

Author affiliations

  • 1 Medical Oncology, Ramathibodi Hospital, 10400 - Bangkok/TH
  • 2 Pathology, Ramathibodi Hospital, 10400 - Bangkok/TH
  • 3 Internal Medicine, Ramathibodi Hospital, 10400 - Bangkok/TH
  • 4 Radiation Oncology, Ramathibodi Hospital, 10400 - Bangkok/TH
  • 5 Molecular Biology, Ramathibodi Hospital, 10400 - Bangkok/TH


Abstract 2839


Early phase clinical studies showed activities of anti PD-L1/PD-1 antibody in head and neck squamous cell carcinomas (HNSCC). PD-L1 expression was extensively evaluated as a biomarker though no standardized method of detection has been defined. HPV status is a well-established prognostic maker of HNSCC. Prevalence, pattern, and impact of PD-L1 expression in HPV-associated HNSCC remain unclear and may vary in different ethnicity.


HNSCC patients treated at the Ramathibodi Cancer Center between 1/2007 and 12/2013 were identified through the cancer registry database. Patient characteristics, treatments, and survivals were abstracted. Archrival formalin-fixed paraffin-embedded (FFPE) tissues were retrieved for PD-L1 and p16 analyses. PD-L1 expression was evaluated by using an anti-human PD-L1 rabbit monoclonal antibody (clone SP142; Ventana) on an automated staining platform (Ventana). PD-L1 was evaluated on tumor cells (TC) and tumor-infiltrating immune cells (IC), and scored ranging between 0-100%. For overall survival (OS) analysis, specimens were scored as ≥5% on TCs or ICs to define PD-L1 positive. HPV status was determined by p16 immunohistochemistry.


204 HNSCC patients with available FFPE tissues were analyzed. PD-L1 on TCs was expression (≥1%) and highly expressed (≥50%) in 72.2% and 18.4% of patients, respectively. With a definition of PD-L1 ≥5% on TCs or ICs, 79.7% of patients was considered as PD-L1+. No statistically difference in patient characteristics between PD-L1 positive and negative, except age ≥65 was associated with a higher incidence of PD-L1 positive (53.4% vs 21.1%; p = 0.001). Overall, 74 patients (36.3%) was p16+. No association of PD-L1 and p16 was observed (p = 0.116). OS was significantly longer in PD-L1+ patients (34.5 vs 21.5 months; p = 0.044). In subgroup analysis of PD-L1/p16 status, OS was superior in patients with +/ + , +/-, -/-, and -/ + , respectively (67.4 vs 23.6 vs 21.5 vs 15.8 months; p = 0.006).


A high prevalence of PD-L1 expression was observed in HNSCC. PD-L1 expression was a strong prognostic maker for OS, especially in p16+ HNSCC patients. These results support further development of anti PD-L1/PD-1 antibody in HNSCC.

Clinical trial identification

Legal entity responsible for the study

Ramathibodi hospital


Ramathibodi Cancer Center


All authors have declared no conflicts of interest.

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