We focused on Calreticulin (CALR) on the basis of proteomic differential display analysis data using the regressive murine fibrosarcoma cell clone QR-32 and the progressive malignant tumor cell clone QRsP-11, derived from QR-32. CALR is an endoplasmic reticulum luminal Ca2 + -binding chaperone protein. CALR is thought to affect the tumor behavior of various malignancies. The purpose of this study was to determine whether CALR expression could be a useful prognostic factor in patients with oral squamous cell carcinoma (OSCC).
CALR expression was investigated by immunohistochemistry in tissue samples from 111 patients with OSCC. The association between CALR expression and clinicopathological characteristics, and patient survival were analyzed.
Immunohistchemical staining of CALR was observed in the cytoplasm of the cancer cells. Among 111 OSCC patients, high expression of CALR was observed in 44 patients (39.6%), whereas 67 patients (60.4%) showed low expression of CALR. Significant association was found between CALR expression and T classification (p = 0.0027), N classification (p = 0.0219), stage (p = 0.0013), and patient outcome (p = 0.0014). The 3-year survival rates of patients with CALR high- and low-expression tumors were 50.1% and 86.6% respectively, which was significantly different (p
These results suggest that elevated expression of CALR might play an important role in the progression of OSCC and could be considered as a useful prognostic factor in patients with OSCC.
Clinical trial identification
Legal entity responsible for the study
Yamaguchi University Graduate School of Medicine
Grant-in-Aid from the Japanese Ministry of Education, Science and Culture
All authors have declared no conflicts of interest.