Epithelial-mesenchymal transition (EMT) is an obligatory event during invasion and metastasis and contributes to tumor progression. Intratumor morphological heterogeneity of invasive carcinoma no special type (IC NST), represented by five types of morphological structures: tubular, trabecular, solid, alveolar structures, and discrete groups of tumor cells, is associated with tumor progression. The presence of alveolar structures in the tumors of untreated patients is linked with lymph node metastasis (Zavyalova et al., 2013), however the mechanism of this fact is unknown. The aim of this work was to estimate the association between morphological structures and metastasis frequency in the chemotherapy treated patients and to study gene expression profile of the structures.
438 IC NST patients (mean age 50, T1-4N0-3M0-1) treated with neoadjuvant chemotherapy have been enrolled. SurePrint G3 v2 8x60K gene expression arrays were used to analyze transcriptome of morphological structures, isolated from fresh tumors (n = 3) by laser microdissection.
Patients with alveolar or trabecular structures or discrete group of cells in breast tumors more frequently displayed lymph node metastasis than cases without these morphological variants (65.3% vs. 33.2%, р
Five types of morphological structures of IC NST may represent different EMT states and thus contribute to tumor metastasis. Research is supported by Russian Science Foundation Grant: No. 14-15-00318
Clinical trial identification
Legal entity responsible for the study
Tomsk Cancer Research Institute
Russian Science Foundation Grant: No. 14-15-00318
All authors have declared no conflicts of interest.