Abstract 68P
Background
The implementation of liquid biopsy for biomarker testing and response to treatment monitoring requires the development of automated methods for circulating free DNA (cfDNA) isolation. Nevertheless, the extraction stage is critical to ensure reliable results.
Methods
The present study compares the MagNA Pure Compact Nucleic Acid Isolation (MPC) Kit I and Maxwell® RSC ccfDNA Plasma Kit (MR) and the later with QIAamp Circulating Nucleid Acid (QCNA) kit using 57 serum samples from cancer patients treated at the Medical Oncology Department at Puerta de Hierro Teaching Hospital in Madrid, Spain.
Results
Firstly, we observed that MPC method significantly extracted less cfDNA than MR (P
Conclusions
Methods for isolation of cfDNA can affect DNA yield and molecular weight fractions recovery. These observations should be taken into account for cfDNA analysis in routine clinical practice in cancer patients.
Clinical trial identification
Legal entity responsible for the study
Hospital Puerta de Hierro Mjadahonda
Funding
Instituto de Salud Carlos III
Disclosure
All authors have declared no conflicts of interest.
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