250P - Circular RNA profile identifies circPVT1 as a proliferative factor and prognostic marker in gastric cancer

Date 17 December 2016
Event ESMO Asia 2016 Congress
Session Poster lunch
Topics Gastric Cancer
Translational Research
Presenter Jie Chen
Citation Annals of Oncology (2016) 27 (suppl_9): ix68-ix85. 10.1093/annonc/mdw582
Authors J. Chen
  • Department Of Gastric Cancer And Soft Tissue Sarcomas, Shanghai Cancer Center Fudan University, 200032 - Shanghai/CN

Abstract

Background

Circular RNAs (circRNAs) comprise a novel class of widespread non-coding RNAs that may regulate gene expression in eukaryotes. However, the characterization and function of circRNAs in human cancer remain elusive. The aim of this study was to assess the circRNA profile and to identify the functional and prognostic significance of circRNA in gastric cancer (GC).

Methods

Ribosomal-depleted RNA sequencing and a bioinformatic analysis were performed to identify circRNA candidates. The expression of circRNA was examined in paired normal and cancerous gastric tissues. Molecular and cellular techniques were used to explore the biological function and mechanism of circRNA in GC cells. The prognostic significance was analyzed using the Kaplan-Meier method and the Cox proportional hazards model.

Results

We identified at least 5500 distinct circRNA candidates and a series of circRNAs that are differentially expressed in GC tissues compared with matched normal tissues. We further characterized one circRNA derived from the PVT1 gene, which was termed circPVT1. The expression of circPVT1 is often upregulated in GC tissues due to the amplification of its genomic locus. circPVT1 may promote cell proliferation by acting as a sponge for members of the miR-125 family. The level of circPVT1 was observed as an independent prognostic marker for overall survival and disease-free survival of patients with GC.

Conclusions

Our findings suggest that circPVT1 is a novel proliferative factor and prognostic marker in gastric cancer.

Clinical trial indentification

12/18/2016

Legal entity responsible for the study

N/A

Funding

NSFC china

Disclosure

All authors have declared no conflicts of interest.