107P - Assessment of correlation between polymorphism of leptin gene promoter (LEP G2548) with its serum levels in patients and controls

Date 18 December 2016
Event ESMO Asia 2016 Congress
Session Poster lunch
Topics Breast Cancer, Locally Advanced
Translational Research
Presenter Zahra Tahmasebi Fard
Citation Annals of Oncology (2016) 27 (suppl_9): ix30-ix34. 10.1093/annonc/mdw576
Authors Z. Tahmasebi Fard
  • Biology, Islamic Azad University, 3973188981 - Tehran/IR

Abstract

Background

The hormone leptin is produced by fat cells and to help the regulation of energy balance by inhibiting hunger. Fat reserves are a set of actions of leptin. Obesity is associated with increased mortality in a range of cancers, including hepatocellular carcinoma, colon cancer, breast cancer, ovarian cancer and prostate cancer. This communication mechanism is not known so far. In this study, were studied the relationship between nucleotide changes LEP Rs 7799039 (-G2548A) in the leptin gene and concentration of leptin in serum.

Methods

For this study, were selected 158 patients with breast cancer and 158 healthy controls. Then, serum and DNA of samples was extracted from blood. All samples were amplified and partially of PCR products were digested by Hha I restriction enzymes until to determine the genotype of individuals. In addition, serum leptin concentrations in both groups were assessed by ELISA technique. At the end of the study were used SPSS 23 software for analysis of data.

Results

Between the two groups was seen statistically significant relationship for age (p = 0.001); body mass index (p = 0.000); smoking (p = 0.026) and serum leptin concentrations (p = 0.001). The risk of breast cancer in patients with mutant genotypes AA was detected 1.68 times higher than those without this genotype (p value= 0.036, OR: 1.686, CI95%: 1.033-2.753). But GG genotype showed a protective effect against cancer (p value= 0.005, OR: 0.523, CI95%: 0.333-0.821). Also for heterozygous genotype was seen 1,508 times higher than other people's (p value= 0.202, OR: 1.508, CI95%: 0.800-2.841). But none of the genotypes was not associated with serum leptin concentration.

Conclusions

Mutant allele A of LEP G2548 polymorphism was associated with breast cancer risk. But this polymorphism did not show the statistical relationship with serum leptin concentrations.

Clinical trial indentification

0

Legal entity responsible for the study

N/A

Funding

Islamic Azad University

Disclosure

All authors have declared no conflicts of interest.