85P - Correlation of intratumoral CD8(+) T-cells, neutrophils and eosinophils frequency with morphological characteristics and clinical outcome of gastro...

Date 10 October 2016
Event ESMO 2016 Congress
Session Poster display
Topics Gastrointestinal Cancers
Presenter Natalia Beliak
Citation Annals of Oncology (2016) 27 (6): 15-42. 10.1093/annonc/mdw363
Authors N. Beliak1, S. Kutukova1, G. Manikhas2, G. Raskin3
  • 1Chemotherapy, City clinical oncology dispensary, 198255 - Saint Petersburg/RU
  • 2Head Physician, City clinical oncology dispensary, 198255 - Saint Petersburg/RU
  • 3Pathology, Russian scientific center of radiology and surgical technology, 197758 - Saint Petersburg/RU

Abstract

Background

The purpose of our study was to investigate some correlation between tumor and microenvironment infiltration of CD8(+) T-cells, eosinophils and neutrophils, clinical parameters, treatment options and OS in our patient cohort.

Methods

In the prospective study we analyzed cards and tumor samples of 15 patients with colorectal and gastric cancer: 6 patients with gastric cancer: 2pp. - stage II; 4pp. - stage IV; 9 patients with CRC: 5pp. - stage III, 4pp. - stage IV. All patients were treated under the standard clinical complex protocol. All patients were under our supervision from 2013 to 2015. We studied clinical stage, level of expression of CD8(+) T-cells, eosinophils and neutrophils in the tumor and microenvironment.

Results

The frequency of neutrophils in microenvironment varied from 0 to 1000 cells/mm2 (median 55.5 cells, 95% CI from 20.55 to 703.61). The frequency of eosinophils varied from 0 to 32 cells/mm2 (median 10 cells, 95% CI from 5.38 to 26.78). Expression of CD8(+) T-cells varied from 26 to 624 cells/mm2 (median 241.5 cells, 95% CI from 117.30 to 379.05). Neutrophil frequency had an inversely correlation with regional lymph nodes (r= -0.84, 95% CI from -0.97 to -0.34, p = 0,0087). Expression of CD8(+) T-cells had inverse correlation with tumor differentiation (r= -0.60, 95% CI from -0.89 to 0.05, p = 0.0682). OS was 27 month (95% CI from 18,00 month, all patients alive on Dec,2015). Cox's regression showed dependence of OS on the frequency of CD8(+) T-cells: p = 0.56, 95% CI of Exp(b) from 0.99 to 1.00); from eosinophils frequency - p = 0,24, 95% CI of Exp(b) from 0.97 to 1.13); from neutrophils frequency - p = 0,89, 95% CI of Exp(b) from 0.99 to 1.00).

Conclusions

Definition of intratumoral expression of CD8(+) T-cells and infiltration of eosinophils and neutrophils could be informative prognostic factors for patient with gastrointestinal adenocarcinomas.

Clinical trial identification

Legal entity responsible for the study

Kutukova Svetlana

Funding

N/A

Disclosure

All authors have declared no conflicts of interest.