15P - Change of mTOR, p6, NBR1 and LC3A/B-I/II in A549 cells treated with graphene oxides

Date 15 April 2016
Event European Lung Cancer Conference 2016 (ELCC) 2016
Session Poster lunch
Topics Cancer biology
Thoracic malignancies
Basic Scientific Principles
Presenter JONG WOOK Shin
Citation Journal of Thoracic Oncology (2016) 11 (supplement 4): S57-S166. S1556-0864(16)X0004-4
Authors J.W. Shin, S.Y. Kim, K.S. Choi, J.W. Jung, J.C. Choi, J.Y. Kim, C.S. Park, I.W. Park, B.W. Choi
  • Department Of Internal Medicine, Chung-Ang University Hospital, 155-755 - Seoul/KR

Abstract

Background

The graphene, an allotrope of carbon, has the honeycombing structure of one-atom-thick planar sheets, is widely used as functional materials for extracellular matrix, drug delivery system, scaffolds for cell growth. This study was designed to check the cellular effects, especially focusing on the autophagy in A549 cells, a lung adenocarcinoma cell line, in response to various forms of graphene nanomaterials. This study reported the change of p62/SQSTM1, NBR1 and LC3A/B-I/II composing the autophagosome machinery.

Methods

A549 cells were cultured in DMEM (Dulbecco's modified eagle's medium) with 10% fetal bovine serum and treated with graphene oxide (GO), dodecyl amine GO(DA-GO), sodium dodecyl sulphate rGO(SGO) and reduced GO(rGO) whose concentrations were 5–200 ug/ml for 24 and 48 hours. Light microscopic features and MTT assay results were obtained. Protein expression was checked by Western blots for LC3A/B-I, II, NBR1 and p62/SQSTM1.

Results

Starvation of serum and overconfluence of cell populations induced the autophagy in adenocarcinoma cells. GAPDH was constant to graphene treatment in adenocarcinoma cells but not actin. Morphologic changes were most prominent in DAGO-treated cells. DA-GO showed the induction of autophagy in concentration-dependent manner. The expression of mTOR, phosphorylated mTOR was decreased by DA-GO. NBR and p62/SQSTM1 were decreased in 24 hours according to increasing concentration of DA-GO in 24 hours but increased in 48 hours treatment of DA-GO. In case of GO, SGO, RGO, the changing patterns showed more complicated patterns.

Conclusions

Taken together, if DA-GO might be used for anticancer drug delivery system, it might be more efficacious for cytotoxicity against lung adenocarcinoma. But further experiment with anticancer drugs must be mandatory for support this anecdotal suggestion.

Clinical trial identification

Legal entity responsible for the study

This research was supported by Mid-career Research Program (2011–0028752) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology.

Funding

This research was supported by Mid-career Research Program (2011–0028752) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology.

Disclosure

All authors have declared no conflicts of interest.