131P - A comparative study on cytotoxicity of nanomaterials: decreased cell viability induced by carbon nanotubes and graphen oxide in HepG2 cell line

Date 20 December 2015
Event ESMO Asia 2015 Congress
Session Poster presentation 2
Topics Cancer biology
Basic Scientific Principles
Presenter shaghayegh Derakhshani
Citation Annals of Oncology (2015) 26 (suppl_9): 37-39. 10.1093/annonc/mdv521
Authors S. Derakhshani
  • Gastroenterlogy And Liver Diseases Research Center, Research Institute For Gastroenterology And Liver Diseases, Shahid Beheshti University of Medical Sciences, 1985711151 - Tehran/IR

Abstract

Aim/Background

The usage of nanotechnology products and mass production of engineered nanomaterials in medicine and biology have been raised in recent years. Carbon nanotubes (CNTs) and Graphen Oxide (GO) are among the widely proposed nanomaterials for application in medicine and industry due to their unique physicochemical properties. The aim of this study was to find the probable toxicity while using such materials as drug delivery vehicles or scaffolds. Besides, the probable method for destructing cancer cells by these materials is considered.

Methods

Investigate cytotoxic effects of CNTs and GO on hepatic cell line (HepG2), that were allowed to differentiate for 14 days after confluency were used for this experiment. CNTs and GO at five different concentrations of 5, 10, 20, 50 and 100 µg/ml were exposed to the cells for 24 hours. The untreated cells serve as control in both experiments. After exposure the XTT assay was used to determine cytotoxic effect of each concentration on the grown cells.

Results

The results revealed that treatment of HepG2 cells for 24 hours with various doses of CNTs and GO decreased the cell viability in a dose-dependent manner. No significant difference in cytotoxic effects was observed between the two components.

Conclusions

Considering the vast usage and promising applications of nanomaterials and increase exposure of human with nanomaterials, the importance of toxicological studies in recent years could be highlighted more than anytime. Furthurmore, the probable ability of CNTs and GOs for destructing cancer cells should be taken into account. However, some studies have used the intrinsic near-IR(NIR) light absorption property of CNTs in order to destruct cancer cells in vitro. More studies are needed to unravel exact mechanism of the interaction of CNTs and GO with hepatic cell line.

Clinical trial identification

Disclosure

All authors have declared no conflicts of interest.