34P - The expression and functional role of Cripto-1 in human colorectal cancer

Date 17 December 2016
Event ESMO Asia 2016 Congress
Session Poster lunch
Topics Colon and Rectal Cancer
Rectal Cancer
Translational Research
Presenter Sato Jun
Citation Annals of Oncology (2016) 27 (suppl_9): ix9-ix18. 10.1093/annonc/mdw574
Authors S. Jun1, H. Karasawa1, T. Suzuki2, S. Nakayama1, M. Katagiri1, S. Maeda1, S. Ohnuma1, F. Motoi1, T. Naitoh1, M. Unno1
  • 1Department Of Surgery, Tohoku University Graduate School of Medicine, 980-8574 - Sendai/JP
  • 2Department Of Pathology & Histotechnology, Tohoku University Graduate School of Medicine, 980-8574 - Sendai/JP

Abstract

Background

Cripto-1 is located in lipid rafts regions of the plasma membrane binding to a glycosylphosphatidylinositol (GPI) anchor and highly expressed in embryonic stem cells. The expression is very low or absent in the adult tissue, whereas Cripto-1 is re-expressed in a variety of human tumors. However, Cripto-1 expression and its function in colorectal cancer have been investigated in several studies, the relationships between Cripto-1 expression and prognosis have not been adequately assessed in colorectal cancer. This study is aimed to evaluate the clinical and biological significance of Cripto-1 in colorectal cancer.

Methods

Colorectal cancer specimens were obtained from 192 patients. The Cripto-1 expression was evaluated by immunohistochemistry and clinicopathological features were statistically analyzed. Functions of Cripto-1 were investigated using colorectal cancer cell lines transfected with Cripto-1 siRNA in vitro and Cripto-1 stable knockdown colorectal cancer cell lines in vivo.

Results

In clinicopathological analysis, the number of Cripto-1-positive cases was 68 out of 195 (35%) in this study. The Cripto-1 status was significantly associated with tumor size (P 

Conclusions

These results suggest that Cripto-1 has potential for a prognostic marker and therapeutic target in human colorectal cancer.

Clinical trial indentification

Legal entity responsible for the study

Tohoku University Graduate School of Medicine

Funding

Japan Society for the Promotion of Science

Disclosure

All authors have declared no conflicts of interest.