1593P - Differential expression of microRNAs and their target genes in non-small-cell lung cancer

Date 28 September 2014
Event ESMO 2014
Session Poster Display session
Topics Translational Research
Non-small-cell lung cancer
Basic Principles in the Management and Treatment (of cancer)
Presenter Hui-Young Lee
Citation Annals of Oncology (2014) 25 (suppl_4): iv546-iv563. 10.1093/annonc/mdu358
Authors H. Lee, S. Han, S. Song, K. Lim, W.J. Kim
  • Internal Medicine, School of Medicine, Kangwon National University, 200-701 - Chuncheon si/KR

Abstract

Aim

MicroRNAs (miRNAs) are single-stranded RNA species that constitute a class of noncoding RNAs that are emerging as key regulators of gene expression. Because each miRNA can regulate multiple genes, miRNAs are attractive markers for studies of coordinated gene expression. We investigated miRNA expression profiling using a massively parallel sequencing technique to compare non-small-cell lung cancer tissue (NSCLC) and normal lung tissue.

Methods

Lung cancer tissue and normal lung tissue were obtained from nine NSCLC patients. RNA isolated from these samples was processed using RNA sequencing (RNA Seq) and the HiSeq 2000 system. Differentially expressed miRNAs and mRNAs were analyzed using a t-test. We selected target pairs that showed a negative correlation among significantly differentially expressed miRNAs and their putative target mRNAs using miRBase Targets.

Results

The differences in the expression levels of 222 miRNAs and 1,597 genes were statistically significant, as indicated by an absolute fold change ≥1.5 and p<0.05. miR-577, miR-301b, miR-944, miR-891a and miR-615-3p were generally upregulated, and miR-338-3p was generally downregulated. miRNA-mRNA target pair analysis revealed that 49 miRNAs had 696 target mRNAs.

Conclusions

There were significantly differentially expressed miRNAs and mRNAs between lung cancer and normal tissue. Further investigation of miRNAs and their target genes is warranted to better understand NSCLC.

Disclosure

All authors have declared no conflicts of interest.