122P - CXCL-12 is a potential marker to predict risk of lymph node metastasis in infiltrating duct carcinoma

Date 18 December 2016
Event ESMO Asia 2016 Congress
Session Poster lunch
Topics Breast Cancer, Metastatic
Translational Research
Presenter DIDIK Heriyanto
Citation Annals of Oncology (2016) 27 (suppl_9): ix35-ix41. 10.1093/annonc/mdw577
Authors D.S. Heriyanto, I. Safitri, J. Purwani, N.A. Setyawan, I. Widodo
  • Anatomic Pathology, Gadjah Mada University/Dr. Sardjito General Hospital, 55281 - Yogyakarta/ID



Prognosis of Breast Carcinoma (BC) depends on several important factors include lymph node status, histological grade, as well as HER2 status. Chemokine receptor CXCR 4 with its ligand CXCL 12, has important role in BC invasiveness. The role of CXCL 12 in BC metastasis and its correlation with prognostic factors is still unclear. Therefore, the study investigates the mRNA level of CXCR 4 and HER2 genes in primer tumor, as well as CXCL 12 gene in lymph node of patiens with and without metastasis and its correlation between each prognostic factor, is needed.


This study involved 50 tissues, diagnosed as infiltrating ductal carcinoma of NST. Group was divided into no metastasis and metastasis. Total RNA was extracted from formalin fixed paraffin embadded. The CXCL 12, CXCR 4 and HER2 mRNA expression were analyzed using quantitative Real Time-PCR. Statistical analysis was performed using Mann-Whitney test and its correlation between each prognostic factor was analyzed using Spearman correlation test.


CXCL 12 expression was significantly higher in patients with metastasis compared to those without metastasis BC (p = 0.007), however, CXCR 4 and HER2 expressions were not significantly difference in primary BC with and without lymph node metastasis. Statistic analysis revealed significant correlation between CXCL 12 expression and poorly grade tumor in metastasis (p = 0.023; r = 0.453), compare to those without metastasis, and CXCR 4 expression was significantly correlated with HER2 in both groups (p = 0.025; and p = 0.003). There was no significant correlation between CXCL 12 and CXCR 4 mRNA expression, as well as HER2 in both groups. However, CXCL 12 expression was significantly correlated with both groups (p = 0.005) compare to those without metastasis.


This study sugested that increase of CXCL 12 expression may have role as prognostic factor for lymph node metastasis and poorly histological grade of BC. The increased level of CXCR 4 mRNA is correlated with HER2 expression in primary tumor of BC.

Clinical trial indentification

Legal entity responsible for the study

Didik Setyo Heriyanto


Universitas Gadjah Mada


All authors have declared no conflicts of interest.