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RT-PCR detects NTRK gene fusions expressed at the RNA level [1]. RT-PCR detects a single fusion if the involved exons of both genes are known. However, multiple transcripts can be covered with a type of RT-PCR known as nested RT-PCR.


  1. Argani P, Fritsch M, Kadkol SS et al. Detection of the ETV6-NTRK3 chimeric RNA of infantile fibrosarcoma/cellular congenital mesoblastic nephroma in paraffin-embedded tissue: application to challenging pediatric renal stromal tumors. Mod Pathol 2000; 13: 29-36.

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Last update: 24 June 2019