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Fluorescence In Situ Hybridisation

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Advantages

The advantages of FISH for detecting NTRK gene fusions are summarised in the following table.

Advantages of FISH for Testing for NTRK Gene Fusions

Advantages

Relatively quick
Does not require much tumour tissue or high tumour content for a single test [1]
Usually high specificity and sensitivity for DNA level rearrangements within a single given gene [2]
Can be performed on many types of biopsy specimens including [1] formalin-fixed paraffin-embedded tissue sections [3, 4]

References

Savic S, Bubendorf L. Common Fluorescence In Situ Hybridization Applications in Cytology. Arch Pathol Lab Med 2016; 140: 1323-1330.
Cui C, Shu W, Li P. Fluorescence In situ Hybridization: Cell-Based Genetic Diagnostic and Research Applications. Front Cell Dev Biol 2016; 4: 89.
Choi Y, Won YJ, Lee S et al. Cytoplasmic TrkA Expression as a Screen for Detecting NTRK1 Fusions in Colorectal Cancer. Transl Oncol 2018; 11: 764-770.
Ou SH. Crizotinib: a novel and first-in-class multitargeted tyrosine kinase inhibitor for the treatment of anaplastic lymphoma kinase rearranged non-small cell lung cancer and beyond. Drug Des Devel Ther 2011; 5: 471-485.