Analysis of microRNA expression in Gastroenteropancreatic Neuroendocrine Tumor

Date 29 June 2016
Event ESMO World Congress on Gastrointestinal Cancer 2016
Session ESMO World Congress on Gastrointestinal Cancer 2016 - Abstracts book
Presenter S.J. Vega Neira
Citation Annals of Oncology (2016) 27 (2): 1-85. 10.1093/annonc/mdw199
Authors S.J. Vega Neira1, M.M. Torres Carvajal1, L. Barrera Herrera2, R. Andrade Perez2, R. Lopez Panqueva2
  • 1Facultad de Ciencias, Universidad de los Andes, Bogotá, Colombia, /
  • 2Department of Pathology, Hospital Universitario Fundación Santa Fé de Bogotá, Bogotá, Colombia, /

Abstract

Gastroenteropancreatic Neuroendocrine tumors (GEP-NETs) are a heterogeneous and rare group of neoplasms arising from epithelial endocrine cells, located along the gastrointestinal tract and corresponding to 0.5% of all tumors affecting the world's population. The molecular basis of GEP NETs involve cell signaling pathways (PI3K/AKT/mTOR, Notch1 and Wnt) which are activated constitutively in 80% of cases. Genes involved in these pathways (HES1, NOTCH1, mTOR, VEGFR2, PDGFRβ and ATRX) have been recognized as prognostic biomarkers. Epigenetic factors like microRNAs (miRNAs) could be involved in post-transcriptional regulation, thus contributing to the development of cancer. Utilizing a bioinformatic approach and the data bases TarjetScanHuman, miranda, miRDB and PicTar we selected a group of miRNAs that might regulate the expression of the biomarkers. For the selection of miRNAs the following criteria were handled 1) chance of joining by homology of microRNA with the major target mRNA up to 80%, 2) that the target mRNA has been predicted in more than 3 databases and 3) previous studies in GEP-NET have reported significant abnormal expression of the miRNA. The miRNAs candidate's chosen were miR-19a, miR-96, miR-145, miR-182 and miR-200a. Objective: Determine the profile of differential expression of these microRNAs in GEP-NETs, in tumor and adjacent normal tissue.