92P - Gastric Cancer Stem-Like Cells, The Balance Operator of Th17 And Treg. (92P)

Date 08 December 2017
Event ESMO Immuno-Oncology Congress 2017
Session Lunch & Poster Display session
Topics Breast Cancer
Presenter Alaleh Rezalotfi
Citation Annals of Oncology (2017) 28 (suppl_11): xi6-xi29. 10.1093/annonc/mdx711
Authors A. Rezalotfi1, M. Ebrahimi2, G. Solgi1
  • 1Immunology, Hamadan University of Medical Sciences, 6517838695 - Hamadan/IR
  • 2Stem Cells And Developmental Biology, ROYAN INSTITUTE FOR STEM CELL BIOLOGY AND TECHNOLOGY, 16635-148 - Tehran/IR



CD4+CD25+FOXP3+ regulatory T (Treg) cells are immune suppressive cells and are prominent with tumor progression. In opposite, CD4+IL-17+ inflammatory T helper 17 (Th17) cells play a critical role in autoimmune disease along with Th1. It is demonstrated that cancer stem-like cells, a rare pluripotent population among tumor cells mediate immune suppressive effects and are responsible for the most tumor progression. Although Treg and Th17 cells have been reported in the most advanced solid tumors, their interaction with gastro-spheres have been elusive. The object of this study was to find the effect of gastro-spheres on differentiation of Treg and Th17 cells.


Gastro-spheres were used as the model for enriched cancer stem like cells. The mixed leukocyte reaction was performed in the presence of MKN-45 cells and its derived gastro-spheres conditioned medium. The cultures were continued for 5 days at 37˚C with stimulation three times. Expansion rate of responder cells was evaluated by CFSE and the percentage of CD4+CD25+FOXP3+ cells and CD4+IL-17+ cells were analyzed by flow cytometry.


Flow cytometry phenotypic analysis revealed that there were increases in percentages of CD4+CD25+FOXP3+ Tregs (4.67 vs 1.53) and CD4+IL-17+ Th17 (3.11 vs 0.34) cells in peripheral blood mononuclear cells (PBMCs) treated with gastro-sphere conditioned medium in comparison with non-gastro-sphere conditioned medium. It was also demonstrated that T cell expansion was not significantly decreased in gastro-sphere conditioned medium treatment compared with the parental (non-gastrospheres) and control group.


We concluded that gastro-sphere-soluble factors increase the percentage of Treg and Th17 cells more than its parental soluble factors. This suggested the possible existence of differentiation factors such as key cytokines among tumor cell secretions, which contribute to T cell differentiation. While it has been shown that gastro-sphere secretions can be possibly more immunomodulator than its parental cell secretions and lead to limited T cell expansion.

Clinical trial identification

Legal entity responsible for the study

Royan Institute for Stem Cell Biology and Technology and Hamadan University of Medical Sciences


Royan Institute for Stem Cell Biology and Technology and Hamadan University of Medical Sciences


All authors have declared no conflicts of interest.