HPV E6/E7 oncoprotein promotes cervical cancer by promoting miR-466 expression via P53 pathway

Date 24 November 2018
Event ESMO Asia 2018 Congress
Session Poster display - Cocktail
Topics Cervical Cancer
Translational Research
Presenter Yong Shen
Citation Annals of Oncology (2018) 29 (suppl_9): ix79-ix86. 10.1093/annonc/mdy436
Authors Y. Shen, Q. Xu
  • Clinical Laboratory, Affiliated Cancer Hospital of Zhengzhou University ﹠ Henan Cancer Hospital, 450008 - Zhengzhou/CN

Abstract

Background

Cervical cancer is the second most common gynecologic cancer among women worldwide. Human papillomavirus (HPV) has been recognized as necessary cause of cervical carcinogenesis. HPV E6/E7 proteins inactivate growth inhibitors within the cell, most importantly the tumor suppressors, p53 and pRB. The potential of miRNAs as novel biomarkers for cervical cancer is growing.

Methods

Fresh cervical tissues were obtained from 225 patients with cervical cancer, cervical intraepithelial neoplasia (CIN), and healthy controls, and the tissues were immediately frozen in liquid nitrogen until use. The RNA was extracted and quantitative real-time polymerase chain reaction (PCR) was performed.

Results

The expression levels of miRNA-466 was significantly increased in CIN group and in cervical cancer group compared with healthy controls group. Furthermore, compared with the expression levels of miRNA-466 in different CIN tissues, the expression levels of miRNA-466 in CIN2 and CIN3 tissues were gradually higher than those in CIN1 tissue. But there was no significant change in the expression of miRNA-466 between CIN2 and CN3 tissue. The level of miRNA-466 in HPV-infected cervical tissue was significantly higher than that in the uninfected tissue. By stratifying the cervical tissue according to grades of cervical lesions, the expression of miRNA-466 in the HPV-infected group was significantly higher than that in the uninfected group in the healthy controls group and CIN group. After the expression plasmid of HPV E6/E7 was stably transfected into SiHa cells for 48 hours, the levels of miRNA-466 and P53 protein in the transfection group were significantly higher than those in the blank group and the negative group.

Conclusions

HPV E6/E7 oncoprotein probably promotes miRNA-466 expression via P53 pathway. HPV E6/E7 oncoprotein was useful for early detection for cervical cancer and indicators for advanced stage and prognosis of the disease.

Editorial acknowledgement

Clinical trial identification

Legal entity responsible for the study

Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital.

Funding

Nursery Fund (3101030102).

Disclosure

All authors have declared no conflicts of interest.