48P - Inhibition of LAP2α can suppress cell aggressiveness in breast cancer

Date 17 December 2016
Event ESMO Asia 2016 Congress
Session Poster lunch
Topics Translational Research
Presenter Xiaofen Li
Citation Annals of Oncology (2016) 27 (suppl_9): ix9-ix18. 10.1093/annonc/mdw574
Authors X. Li1, Y. Huang2, Y. Hu3
  • 1Medical Oncology, 2nd Affiliated Hospital of Zhejiang University University School of Medicine, 310009 - Hangzhou/CN
  • 2Cancer Institute, 2nd Affiliated Hospital of Zhejiang University University School of Medicine, 310009 - Hangzhou/CN
  • 3Surgical Oncology, 2nd Affiliated Hospital of Zhejiang University University School of Medicine, 310009 - Hangzhou/CN

Abstract

Background

Breast cancer is the most common malignancy in women worldwide. But to date, there is no good serum protein marker for breast cancer diagnosis and prognosis. We conducted this study to screen and identify specific protein markers of breast cancer.

Methods

Mass spectrometry was applied to screen and identify differential serum protein profiles between 63 breast cancer patients and 40 healthy controls. Immunohistochemistry (IHC) and western blot analysis were used as verification experiments. Biological functions of candidate protein marker were studied in vitro.

Results

Two amino acid fragments with sizes of 3.9kDa and 5.6kDa, respectively, were detected as candidate markers. A combined diagnostic model composed of these two fragments was built, with a sensitivity of 82.3% and a specificity of 95.3%. The 5.6kDa fragment, which was up regulated in breast cancer, was an important segment of lamina-associated polypeptide 2 alpha (LAP2α). IHC and western blot experiments confirmed over expression of LAP2α in breast cancer tissues. An inhibition plasmid vector of LAP2α-small hairpin RNA (shRNA) was constructed and transfected into MCF-7 cells. CCK-8 experiments on transfected cells showed that inhibition of LAP2αcould not influent cell proliferation. Transwell and matrigel-transwell assays indicated that inhibition of LAP2α could significantly reduce cell migration and invasion abilities.

Conclusions

We built a diagnostic model for early detection of breast cancer with a satisfactory accuracy. LAP2αwas identified as a candidate serum protein marker, which was overexpressed in breast cancer. Inhibition of LAP2αcould suppress MCF-7 cell aggressiveness. Our study indicated that LAP2αcould be a potential serum biomarker and therapeutic target of breast cancer.

Clinical trial indentification

none

Legal entity responsible for the study

The Second Affiliated Hospital, Zhejiang University School of Medicine, China

Funding

Zhejiang Provincial Natural Science Foundation of China

Disclosure

All authors have declared no conflicts of interest.