33P - Pathological features of ALK mutations in Malaysian non-small cell lung cancer (NSCLC) patients

Date 19 December 2015
Event ESMO Asia 2015 Congress
Session Poster presentation 1
Topics Thoracic Malignancies
Pathology/Molecular Biology
Basic Scientific Principles
Presenter ROZIANA Ariffin
Citation Annals of Oncology (2015) 26 (suppl_9): 8-15. 10.1093/annonc/mdv518
Authors R.B. Ariffin1, K.A.B. Daud1, A.R.B. Abdul Muttalif2, N.B. Mohd Marzuki2
  • 1Genetics, General Hospital Kuala Lumpur, 50586 - Kuala Lumpur/MY
  • 2Respiratory Medicine, Institute of Respiratory Medicine(Institut Perubatan Respiratori), 50586 - Kuala Lumpur/MY



We aim to describe the pathological features of ALK positive and negative among Malaysian NSCLC patients.


This is a retrospective study of 83 NSCLC patients with review of histological features. FISH was performed on formalin-fixed, paraffin-embedded (FFPE) tumor tissues using Vysis ALK Break Apart FISH Probe. Signal interpretation was in accordance with College of American Pathologists guidelines.


Nine patients (11.5%) exhibit ALK rearrangements (excluding the uninterpretable cases) 69 patients were negative and 5 patients were not interpretable. Of the 9 ALK-positive cases, 8 (88.9%) were adenocarcinomas, and 1(11.1%) was squamous cell carcinoma. Age of ALK-positive cases ranges from 33 to 71 years with 5(55.6%) male and 4(44.4%) female. Histology range from moderately to poorly differentiated adenocarcinoma. One case showed Squamous cell carcinoma, one case showed papillary adenocarcinoma with TB pleura.


ALK gene rearrangement was seen in 11.5% and ALK positive patients were relatively younger than negative patients. Histology ranges from moderately to poorly differentiate with one case showing squamous cell carcinoma. For uninterpretable result, test report was relayed to requesting clinicians for repeat testing when possible.

Clinical trial identification


All authors have declared no conflicts of interest.