Prognosis of esophageal squamous cell carcinoma based on local immunity evaluation

Date 29 September 2019
Event ESMO 2019 Congress
Session Poster Display session 2
Topics Oesophageal Cancer
Presenter Elena Zlatnik
Citation Annals of Oncology (2019) 30 (suppl_5): v253-v324. 10.1093/annonc/mdz247
Authors E.Y. Zlatnik1, O.I. Kit2, A.L. Bazaev3, I.A. Novikova2, A.A. Demidova4, L.Y. Vladimirova5
  • 1Laboratory Of Immunophenotyping Of Tumors, Rostov Research Institute of Oncology, 344037 - Rostov-on-Don/RU
  • 2Administration, Rostov Research Institute of Oncology, 344037 - Rostov-on-Don/RU
  • 3Department Of Surgical Oncology, Rostov Research Institute of Oncology, 344037 - Rostov-on-Don/RU
  • 4Department Of Medical And Biological Physics, Rostov State Medical University, 344 - Rostov-on-Don/RU
  • 5Medical Department, Rostov Research Institute of Oncology, 344037 - Rostov-on-Don/RU

Abstract

Background

The purpose of the study was to assess the significance of the local cytokine composition in patients with esophageal squamous cell carcinoma (EC) for the disease prognosis.

Methods

Cytokines were studied in tumor, peritumoral (PT) and resection line (RL) tissues of 36 patients with stage II-III EC. All patients received surgery; further treatment depended on the tumor stage. During the observation, patients were divided into groups with short and prolonged progression-free survival (< and >1.5 years). Levels of TNF-α, IL-1b, IL-6, IL-8, and IL-10 were determined in tissue homogenates by ELISA and calculated in pg/mL/g of protein. Discriminant and ROC analyses were performed with the calculation of the area under a ROC curve (AUC) and cut-off points for statistically significant indicators sharing with maximum diagnostic sensitivity (DSe) and specificity (DSp) two alternative signs: the presence or absence of the risk of early progression.

Results

Patients showed different initial levels of tissue pro-and anti-inflammatory cytokines. PT of patients with the further progression was characterized by higher levels of TNF-α (by 1.9) and IL-10 (by 1.6 times), and their tumor tissues – by 2.1 times higher IL-1b and 1.6 times higher IL-8 (p < 0.05). The ROC analysis demonstrated the following cut-off values: in tumors 57.5 pg/mL/g (IL-1b) and 4.5 pg/mL/g (IL-10), in PT 36.0 pg/mL/g (IL-8) and 7.4 pg/mL/g (TNF-α), in RL 9.8 pg/mL/g (TNF-α) and 5.2 pg/mL/g (IL-10). Exceeding them involved the risk of early disease progression. Levels of TNF-α in PT, IL-10 in tumors and RL showed good (AUC 0.8-0.9), and levels of TNF-α in RL, IL-1b in tumors and IL-8 in PT - satisfactory (AUC 0.7-0.8) ability to recognize the risk of early EC progression, p < 0.005.

Conclusions

Evaluation of local levels of cytokines allowed identifying the risk criteria for the development of early progression of EC, which along with clinical signs (the process spread) determine its prognosis.

Clinical trial identification

Editorial acknowledgement

Legal entity responsible for the study

The authors.

Funding

Has not received any funding.

Disclosure

All authors have declared no conflicts of interest.