1554P - The role of Krüppel-like factor (KLF5) and its mechanism for treatment resistance in preoperative chemoradiation therapy for rectal cancer

Date 10 October 2016
Event ESMO 2016 Congress
Session Poster display
Topics Translational Research
Presenter Jeong Yeon Kim
Citation Annals of Oncology (2016) 27 (6): 526-544. 10.1093/annonc/mdw392
Authors J.Y. Kim1, S.G. Park2, N.K. Kim3
  • 1Department Of Surgery, Hallym university College of medicine, 200-161 - Kungki-do/KR
  • 2Department Of Surgery, hallym university, Kungki-do/KR
  • 3Department Of Surgery, Yonsei cancer center, Seoul/KR

Abstract

Background

The aim of this study was to determine whether Krüppel-like factor 5(KLF5) expression in pre-irradiation tumor biopsies is a useful predictive marker of tumor response in patients with rectal cancer

Methods

This study included 60 human colon tumor pre-irradiation specimens. Expression was studied by immunohistochemistry(IHC) using scoring system(0-15). Functional roles of KLF5 were analysed by over-expression of the protein in colon cancer cell line. Protein interactions were studied by stress induction such as chemo or radiation and MTT assays.

Results

Complete remission was achieved by 9(18%) patients. Tumor regression was significantly related with p53 and KLF5(p = 0.021,p = 0.004,respectfully). The KLF5 IHC score significantly correlated with KRAS mutation status(5.92 ± 2.54 vs 8.44 ± 1.94,p = 0.006),and pCR(4.11 ± 2.61 vs 6.68 ± 2.43,p = 0.005). In HCT 116 cell line, KLF5 protein was significantly increased after radiation therapy, suggesting that KLF5 via cyclin D1, b-catenin. HCT 116 with KLF5 overexpression exhibited significantly better cell viability compared to control cells in MTT assay.

Conclusions

Overexpression of KLF5 might be predictive of poor tumor regression after preoperative CRT. Our study suggests IHC of KLF5 as a possible biomarker to predict complete remission and T-down staging. Our study suggests KLF5 has a role to get resistance to chemo-radiation therapy in rectal cancer treated preoperative CRT.

Clinical trial identification

Legal entity responsible for the study

N/A

Funding

N/A

Disclosure

All authors have declared no conflicts of interest.