1598P - Characteristics and prognostic potential of tertiary lymphoid structures in oral tongue squamous cell carcinoma

Date 10 October 2016
Event ESMO 2016 Congress
Session Poster display
Topics Pathology/Molecular Biology
Presenter Xiangqi Liu
Citation Annals of Oncology (2016) 27 (6): 545-551. 10.1093/annonc/mdw393
Authors X. Liu1, Y. Wang2, J. Fang2, J. Song2, Y. Chen2, T. Wu2, J. Wang2, B. Cheng2, Z. Wang2
  • 1Department Of Oral And Maxillofacial Surgery, Guanghua School Of Stomatology, Hospital Of Stomatology, Sun Yat-Sen University, 457 - Guangzhou/CN
  • 2Department Of Oral Medicine, Guanghua School Of Stomatology, Hospital Of Stomatology, Sun Yat-Sen University, Guangzhou/CN

Abstract

Background

Recently, tertiary lymphoid structures (TLSs) have been reported in melanoma, NSCLC, breast cancer, colorectal cancer and so on. They are considered to drive local adaptive immune responses against tumors and considered as important sites of extranodal T cell priming and epitope spreading in the responder T cell repertoire. In terms of prognostic value, TLS is the factory for immune response. Therefore TLS act as a favorable clinical outcome for patients. But TLS was seldom reported on oral tongue squamous cell carcinoma. To identify and evaluate the presence of tertiary lymphoid structures in oral tongue squamous cell carcinomas (OTSCC). If present, we would study the prognostic values of TLSs in OTSCC and analyze whether they are related to the survival of OTSCC.

Methods

The expression of PNAd+ HEV, CD20+ B cells, CD3+ T cells was examined to record the quantity of TLSs, using immunohistochemistry (IHC) in paraffin-embedded tissue samples from 168 OTSCC patients. Overall Survival (OS) and disease-free survival (DFS) were estimated using the Kaplan-Meier method, with a log-rank test. Multivariate survival analysis was done using the Cox proportional hazards model. Variables that were statistically significant in the univariate analysis were entered into multivariate Cox regression analyses to indentify its independent value.

Results

TLS was a well-organized structure composed of a specific indicator of HEV and distinct B cell aggregates and T cell area. TLSs were found in about 26.5% of OTSCC patients. Among clinicopathological data [including gender, age, smoking history, alcohol consumption, tumor differentiation, TNM stage and TILs (tumor infiltrating lymphocyte)], TLSs were associated with TILs (p 

Conclusions

The presence of TLS in OTSCC was identified. TLS is associated with adverse prognosis in patients with OTSCC. It may play important roles in preventing the recurrence of OTSCC.

Clinical trial identification

Legal entity responsible for the study

Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangdong Provincial Key Laboratory of Stomatology

Funding

National Natural Science Foundations of China (No. 81272954, 81472524)

Disclosure

All authors have declared no conflicts of interest.