165P - Ranolazine before and during trastuzumab treatment, prevents cardiotoxicity in mice

Date 28 September 2014
Event ESMO 2014
Session Poster Display session
Topics Cancer Biology
Basic Scientific Principles
Presenter Nicola Maurea
Citation Annals of Oncology (2014) 25 (suppl_4): iv53-iv57. 10.1093/annonc/mdu325
Authors N. Maurea1, C. Coppola2, G. Piscopo2, D. Rea3, F. Galletta2, C. Maurea2, I. Capasso4, C. Arra3, R.V. Iaffaioli5
  • 1Cardiology, INT Pascale Naples, 80131 - Naples/IT
  • 2Cardiology, National Cancer Institute, Pascale Foundation, Naples/IT
  • 3Animal Experimental Research, National Cancer Institute, Pascale Foundation, Naples/IT
  • 4Breast Oncology Division, National Cancer Institute, Pascale Foundation, 80131 - Naples/IT
  • 5Colorectal Oncology, National Cancer Institute, Pascale Foundation, Naples/IT

Abstract

Aim

ErbB2 is overexpressed in about 25% of breast cancers. It modulates myocardial development and function in the heart. Trastuzumab (T), an anti-ErbB2 inhibitor, has improved the prognosis of patients with breast cancer, but is related to an increased risk of asymptomatic left ventricular (LV) dysfunction (3–34%) and heart failure (2–4%). The mechanisms of T cardiotoxicity are not entirely known and can include changes in Ca2+ regulation related to blockade of ErbB2. Here, we aim at assessing whether ranolazine (RAN), diminishing intracellular Ca2+ through its inhibition of late Ina, blunts T cardiotoxicity in vivo.

Methods

To evaluate cardiac function in vivo, fractional shortening (FS) and ejection fraction (EF) were measured by echocardiography M-Mode in C57BL6 mice, 2-4 mo old, pretreated with RAN (750mg/kg/day, a dose comparable to the one used in humans) per os for 3 days. RAN was then administered for additional 7 days, alone and together with T (2.25 mg/kg/day ip), according to our well established protocol.

Results

In our in vivo studies, after 7 days with T, FS decreased to 49 ± 1.5%, p < 0.01 vs 60 ± 0.5% (sham), and EF to 81 + 2%, p < 0.01 vs 91 ± 1% (sham). RAN alone did not change FS (59 ± 2%) nor EF 89 ± 1%. Interestingly, in mice treated with RAN and T, RAN prevents the reduction of EF and FS vs T alone (FS was 58 ± 1%, EF was 90 ± 1%, p = 0.01 and p < 0.01 respectively).

Conclusions

In our mouse model, T produces LV dysfunction and RAN blunts T cardiotoxic effects. We plan to test RAN as a cardioprotective agent with other target-therapy drugs in our experimental models and to define the mechanisms of cardioprotection.

Disclosure

All authors have declared no conflicts of interest.