780P - High testosterone levels in prostate tissue obtained by needle biopsy correlate with poor prognosis factors in prostate cancer patients

Date 27 September 2014
Event ESMO 2014
Session Poster Display session
Topics Prostate Cancer
Translational Research
Basic Principles in the Management and Treatment (of cancer)
Presenter Yasuhide Miyoshi
Citation Annals of Oncology (2014) 25 (suppl_4): iv255-iv279. 10.1093/annonc/mdu336
Authors Y. Miyoshi1, S. Umemoto1, H. Uemura1, Y. Shibata2, K. Sakamaki3, S. Honma4, K. Suzuki5, Y. Kubota1
  • 1Urology, Yokohama City University School of Medicine, 2360004 - Yokohama/JP
  • 2Urology, Gunma University Graduate School of Medicine, 3718511 - Maebashi/JP
  • 3Biostatistics And Epidemiology, Yokohama City University Medical Center, 2320024 - Yokohama/JP
  • 4Biology, ASKA Pharma Medical Co., Ltd., Kawasaki/JP
  • 5Department Of Urology, Gunma University, JP-371-8511 - Maebashi/JP



There has been no consensus on the prostatic tissue androgen concentrations in men with different stages and pathological grades of prostate cancer. In this study, testosterone (T) and dihydrotestosterone (DHT) concentrations in needle biopsy specimens of prostate were examined by newly developed ultra sensitive LC-MS/MS method.


We analyzed 243 men (mean age 71.0 years) diagnosed as prostate cancer. Those patients received systematic needle biopsy, and additionally one needle biopsy from proximal zone was conducted for the purpose of simultaneous determination of T and DHT. T and DHT concentrations in prostate tissues were determined by LC-MS/MS method. Determination limit of this method was 0.5 pg/shot for T and 1 pg/shot for DHT. Concentration of T and DHT were expressed in pg/mg. T and DHT levels in tissue were compared with Gleason score, clinical stage, and percentage of positive biopsy cores (% positive cores) by multivariate analyses.


Median value of serum PSA and prostate volume measured by ultrasound were 11.4 ng/mL and 28.9 cc, respectively. Median values of T and DHT in tissue were 0.49 pg/mg and 6.95 pg/mg, respectively. There was no significant correlation between tissue T and DHT. In multivariate analysis, serum PSA and tissue T levels were significantly associated with poor prognosis factors in men with prostate cancer. High testosterone levels in prostate tissue were related to high Gleason score (GS ≥8 vs ≤7: HR1.491, p = 0.025), advanced clinical stage (stage ≥IV vs ≤III: HR1.027, p = 0.001), and high proportion of % positive cores (≥30% vs <30%: HR1.824, p = 0.002).


Testosterone and dihydrotestosterone concentrations in prostate tissue of needle biopsy were simultaneously examined by newly developed ultra sensitive quantifying method, LC-MS/MS. We confirmed that high testosterone levels in prostate tissue were related to high Gleason score, advanced clinical stage, and high proportion of % positive cores. These results suggest that testosterone level of needle biopsy specimens may be useful as a predictive factor in prostate cancer patients.


All authors have declared no conflicts of interest.