1648P - Functional activity of Ehrlich carcinoma cancer stem cells after treatment by orthovanadate-based nanoparticles

Date 28 September 2014
Event ESMO 2014
Session Poster Display session
Topics Cancer Biology
Basic Scientific Principles
Presenter Anatoliy Goltsev
Citation Annals of Oncology (2014) 25 (suppl_4): iv564-iv573. 10.1093/annonc/mdu359
Authors A.N. Goltsev1, O.V. Chelombit’ko1, N.N. Babenko1, Y.A. Gayevskaya1, T.G. Dubrava1, N.A. Bondarovich1, M.V. Ostankov1, A.Y. Dimitrov1, V.K. Klochkov2, N.S. Kavok2, Y.V. Malyukin2
  • 1Cryopathophysiology And Immunology, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, 61015 - Kharkov/UA
  • 2Nanocrystal Materials, Institute for Scintillation Materials of the National Academy of Sciences of Ukraine, 61001 - Kharkov/UA

Abstract

Aim

Development of tumors results from the expansion of cancer stem cells (CSCs) population. The application of nanocomposites capable of CSCs visualization, binding with them and inducing tumor destruction is a prospective task in oncology. This research aimed to study the CSCs' functional activity as a part of Ehrlich's adenocarcinoma (EAC) after incubation with orthovanadate-based nanoparticles.

Methods

Experiments were implemented in Balb/C mice with experimental induction of EAC. The EAC cells were cultured for 7 days in the peritoneal cavity (PC) of mice prior to and after incubation with nanoparticles (NP) of different forms and concentrations. The methods for procurement of aqueous colloid solutions of orthovanadate-based NP were described [Klochkov V.K., 2009]. Luminescence spectra of EAC cells after incubating with NP were analyzed with monochromator MDR-23. The intensity of EAC development was determined by the volume of ascitic fluid in PC taking cell concentration into account. EAC cells after incubation with NP were visualized in luminescent microscope Olympus IX 71; cells with phenotype CD44hi, CD44+CD24, CD44+CD24+, CD44CD24+, CD117+, Sca-1+ were analyzed with flow cytometer (Facs Calibur); the level of nanog gene expression was assessed with RT-PCR.

Results

According to data from luminescent microscopy only NP of spherical shape demonstrated the capability of visualizing EAC cells. In this case all the types of NP inhibited tumor growth to different extents. The highest inhibition of tumor growth and nanog gene expression was established using spindle-like NP.

Conclusions

We demonstrated here the capability of certain shapes of orthovanadate-based synthesized NP to inhibit EAC growth, that may be further used in clinical practice for therapy of oncological diseases.

Disclosure

All authors have declared no conflicts of interest.