79P - Tiechoic acids from Staphylococcus aureus enhance cytotoxic/cytostatic influence of bimetallic complex on primary tumor culture

Date 15 April 2016
Event European Lung Cancer Conference 2016 (ELCC) 2016
Session Poster lunch
Topics Cancer Biology
Thoracic Malignancies
Basic Scientific Principles
Presenter Viktoriia Nikulina
Citation Journal of Thoracic Oncology (2016) 11 (supplement 4): S57-S166. S1556-0864(16)X0004-4
Authors V. Nikulina1, L. Garmanchuk2, N. Senchylo2, T. Nikolaienko2
  • 1Educational And Scientific Centre “institute Of Biology”, Taras Shevchenko National University of Kyiv, 03022 - Kyiv/UA
  • 2Educational And Scientific Centre “institute Of Biology”, Taras Shevchenko National University of Kyiv, Kyiv/UA

Abstract

Background

Ligands of Toll-like receptors (TLR) are often used as adjuvants in order to enhance the immunogenicity of vaccines in anticancer therapy. Such ligands are cell wall biopolymers of gram-positive microorganisms Staphylococcus aureus – techoic acids (TAs). They play a significant role as immunomodulators. Our previous studies showed that TAs in joint application with bimetallic complex of copper and cadmium with ethylenediamine (PO244) increased antitumor effect of the last.

Methods

In order to determine possible mechanisms of TAs+PO244 impact on tumor through immune cells we studied primary Lewis lung carcinoma (LLC) culture after the impact of macrophages from LLC-bearing mice at the last stage of carcinogenesis. Both TAs and PO244 were administrated on 8th day after tumor cell inoculation. After the therapy macrophages were contactless co-cultivated with primary LLC culture during 48 h. Mononuclear phagocyte fraction from peritoneal exudate of mice was obtained by standard Pietrangeli's procedure. Apoptotic index and distribution of LLC cells in phases of cell cycle were assessed by flow cytometry.

Results

Aforementioned combination revealed in 2-times increasing of LLC cells apoptotic level in comparison with primary LLC cells (without co-culture) and LLC cells under condition of co-cultivation with macrophages from mice without therapy. TAs+PO244 therapy decreased population of LLC cells in proliferative pool (G2/M+S phase) to 40%, whereas control rates were 65% and 60% in LLC cells without co-culture and LLC cells with macrophage co-culture from mice without therapy, respectively.

Conclusions

Cytotoxic/cytostatic influence, which was expressed in increasing of apoptotic level and decreasing of cell population of proliferative pool was defined after co-cultivation of macrophages from LLC-bearing mice treated by TAs+PO244 with primary LLC culture. This effect can be one of the possible mechanisms of TAs+PO244 impact on the tumor.

Clinical trial identification


Legal entity responsible for the study

Taras Shevchenko National University of Kyiv

Funding

Taras Shevchenko National University of Kyiv

Disclosure

All authors have declared no conflicts of interest.