7P - Proangiogenic effect of peritoneal macrophages from animals with Lewis lung carcinoma

Date 15 April 2016
Event European Lung Cancer Conference 2016 (ELCC) 2016
Session Poster lunch
Topics Cancer Biology
Thoracic Malignancies
Basic Scientific Principles
Presenter Tetiana Nikolaienko
Citation Journal of Thoracic Oncology (2016) 11 (supplement 4): S57-S166. S1556-0864(16)X0004-4
Authors T. Nikolaienko1, D. Shelest2, L. Garmanchuk1, O. Dzhus1, V. Nikulina1
  • 1Biochemistry, Educational and Scientific Centre «Institute of Biology» of Taras Shevchenko National University of Kyiv, 03022 - Kyiv/UA
  • 2Biochemistry, Educational and Scientific Centre «Institute of Biology» of Taras Shevchenko National University of Kyiv, Kyiv/UA

Abstract

Background

Both inflammation and angiogenesis are exacerbated by increased production of chemokines/cytokines, growth factors, proteolytic enzymes and prostaglandins. Initiation and progression of cancer are also closely linked to angiogenesis. Infiltration of macrophages is a dramatic and common feature of inflammation, angiogenesis and cancer, and has been recently highlighted in an attempt to develop novel strategies for treating cancer. The recruitment and infiltration of tumor-associated macrophages in the tumor microenvironment activates them to support the malignant progression of cancer cells. The aim of our work is to study the angiogenic potential of macrophages at different stages of growth and metastasis of Lewis lung carcinoma.

Methods

Mononuclear phagocytes obtained from peritoneal exudate of mice with Lewis lung carcinoma. Was carried out co-culturing macrophages with endothelial cells and evaluated their effects on angiogenesis. Cell cycle parameters were assessed by cytoflourometry. The level of VEGFproduction by macrophages was determined by ELISA.

Results

It was shown that cells of the control group passed to S+G2/M by 5.32%, while the number of cells stimulated by incubation medium of peritoneal macrophages on 19 and 25 days passed to S+G2/M increased by 52.82% and 49.72% respectively. It was found increased production of VEGF on 7 days to 2 times, 19 days – 4, 25 days – 4.7 as compared to intact group. Since 19 and 25 days characterized by intensive development of tumors and metestaz, in this time is formed of hypoxic areas. This process induces macrophages to increase production of VEGF for improving the access of oxygen and nutrients in these areas.

Conclusions

The results suggest angiogenic potential of peritoneal macrophages because is observed active transition of endothelial cells to S+G2/M, increased production of VEGF and stimulate the formation of vessel-like structures by endothelial cells.

Clinical trial identification

European equivalent

Legal entity responsible for the study

Educational and Scientific Centre «Institute of Biology» of Taras Shevchenko National University of Kyiv

Funding

Educational and Scientific Centre «Institute of Biology» of Taras Shevchenko National University of Kyiv

Disclosure

All authors have declared no conflicts of interest.