211P - Inflammatory cells characterization and localization in malignant pleural mesothelioma (MPM) tissue samples: Correlation with histologic subtype an...

Date 15 April 2016
Event European Lung Cancer Conference 2016 (ELCC) 2016
Session Poster lunch
Topics Mesothelioma
Translational Research
Presenter Giulia Pasello
Citation Journal of Thoracic Oncology (2016) 11 (supplement 4): S57-S166. S1556-0864(16)X0004-4
Authors G. Pasello1, L. Urso2, V. Polo2, L. Bonanno1, N. Nannini3, F. Lunardi3, S.E. Vuljan3, P.F. Conte2, F. Rea3, F. Calabrese3
  • 1Medical And Experimental Oncology Department, Medical Oncology 2, Istituto Oncologico Veneto IRCCS, 35128 - Padova/IT
  • 2Surgical Oncological And Gastroenterological Sciences Deparment, University of Padova, 35128 - Padova/IT
  • 3Department Of Cardiothoracic And Vascular Sciences, University of Padova, 35128 - Padova/IT

Abstract

Background

Malignant pleural mesothelioma (MPM) is a rare pleural tumor associated with asbestos exposure and subsequent chronic inflammation. Prognosis of MPM is predicted by clinical features and histological subtype: epithelioid (E) is associated with a better treatment outcome and patients prognosis compared with sarcomatoid/biphasic (S/B). The aim of this pilot study is to characterize inflammatory cells in tumor samples from chemonaive mesothelioma samples and to correlate the inflammation immunophenotype with prognosis

Methods

Inflammatory cells, involving intra e peri-tumoral stroma, were characterized by immunohistochemistry using monoclonal anti-CD20 (B lymphocytes), CD3 (T lymphocytes) and CD68 (macrophages) antibodies, and quantified as percentage in neoplastic area examined at low power (4X). The Kruskall-Wallis test was performed to evaluate different inflammatory patterns in the two histological subtypes; correlation studies were performed through Spearman linear correlation analysis; survival curves were designed according to the Kaplan–Meier method

Results

20 MPM chemonaive samples (10 E and 10 S/B) from a homogeneous patient series were analyzed. Higher inflammation was seen in E compared with S/B samples (p = 0.05) both at intra (p = 0.025) and peri-tumoral areas (p = 0.05). B lymphocytes were mainly detected in peri-tumoral area, T lymphocytes and macrophages were present both at the intra-tumoral and peri-tumoral level. Higher peritumoral B and T lymphocytes and intratumoral macrophages were observed in E samples compared with S/B, although not statistically significant. Higher intra-tumoral B lymphocyte infiltrate was correlated with a worse EORTC prognostic score (p = 0.04) and with higher proliferation index (p = 0.05). No statistically significant correlation was observed with other immunophenotypes and clinical data

Conclusions

E and S/B mesothelioma samples are characterized by different inflammation patterns, and these features might impact survival data of affected patients. A confirmatory study on a larger sample size, enriched with cytokines characterization data, is currently ongoing

Clinical trial identification

Not applicable

Legal entity responsible for the study

Istituto Oncologico Veneto

Funding

Istituto Oncologico Veneto

Disclosure

All authors have declared no conflicts of interest.