41P - Analysis of penetration of smokeless tobacco forms using egg shell membrane as a substrate

Date 15 April 2016
Event European Lung Cancer Conference 2016 (ELCC) 2016
Session Poster lunch
Topics Aetiology, Epidemiology, Screening and Prevention
Basic Scientific Principles
Presenter ANOOP Narayanan
Citation Journal of Thoracic Oncology (2016) 11 (supplement 4): S57-S166. S1556-0864(16)X0004-4
Authors A.K. Narayanan
  • Department Of Oral Pathology And Microbiology, P.S.M COLLEGE OF DENTAL SCIENCE AND RESEARCH AKKIKAVU TRICHUR, 680519 - Calicut/IN



The aim of the following study is to determine the particle sizes of smokeless tobacco forms and thereby evaluate the degree of diffusion of the products using an egg shell membrane as a natural substrate by scanning electron microscopy (SEM).


The particle size of smokeless tobacco forms namely mawa, gutka, khaini, and tobacco leaves was determined by image analysis and the products were subjected, on an egg shell membrane, to artificial saliva along with constant grinding force. The processed egg shell membrane was then examined by SEM to evaluate the morphology and the degree of diffusion of these particles.


The morphometric image analysis revealed khaini to be smallest in size followed by mawa, gutka, and tobacco leaves. The control group (egg shell membrane not subjected to any products) under SEM demonstrated intricately woven collagen meshwork, which was regular in length, branched and anastamosed with each other. Khaini exposed membrane demonstrated maximum particle diffusion with disruption of collagen meshwork. Mawa exposed membranes demonstrated minimal particle penetration which were adherent to the collagen meshwork which was irregular with increased interfibrillar space. The egg shell membrane exposed to gutka demonstrated particle penetration at higher magnification with more or less a regular collagen meshwork. The tobacco leaf exposed egg shell membrane demonstrated minimal blebs on the meshwork surface.


The degree of diffusion gradually increased with a decrease in the particle size establishing an inverse relationship

Clinical trial identification


Legal entity responsible for the study

P.S.M College of Dental Sciences and Research Akkikavu Kerala India


P.S.M Dental College India


All authors have declared no conflicts of interest.