67P - mRNA expression level of lymphangiogenesis-associated genes in early-stage non-small cel lung cancer

Date 17 April 2015
Event ELCC 2015
Session Poster lunch
Topics Non-Small-Cell Lung Cancer, Early Stage
Translational Research
Presenter Oksana Kowalczuk
Citation Annals of Oncology (2015) 26 (suppl_1): 18-23. 10.1093/annonc/mdv048
Authors O. Kowalczuk1, M. Kozlowski2, W. Laudanski2, J. Kisluk1, J. Niklinski1
  • 1Clinical Molecular Biology, Medical University of Bialystok, 15-269 - Bialystok/PL
  • 2Clinics Of Thoracic Surgery, Medical University of Bialystok, Bialystok/PL

Abstract

Aim/Background

Although an association between the lymphatic system and metastases has been clearly demonstrated in a number of animal experimental models of cancer, clinical data are much less consistent. The role of the lymphatic system in lung cancer cell dissemination remains uncertain. We examined mRNA expression of the genes encoded for lymphatic-specific growth factors VEGFC, VEGFD, their receptor VEGFR3 and co-receptor NRP2, transcription factors PROX and FOXC2, lymphatic vessel-associated proteins LYVE-1 and podoplanin (PDPN), integrin alpha 9 (ITGA9) and intercellular junction protein desmoplakin (DSP) in stage I-II non-small cell lung cancer (NSCLC). We assumed that an increased activity of the genes would point to the induction of the process of new lymphatic vessel formation through lymphangiogenesis.

Methods

The study was performed on 141 pairs of fresh-frozen surgical specimens of NSCLC cross-sections and matched unaffected lung tissues. Comparative RT-PCR method was used to assess mRNA level.

Results

In the NSCLCs, mRNA expression of the majority of the analyzed genes was similar (PDPN and PROX1, p = 0.640 and p = 0.611, respectively) or significantly lower (VEGFC, VEGFD, VEGFR3, LYVE1, ITGA9, p < 0.0001 for all; FOX2, p = 0.0003 and NRP2, p = 0.021) than that in matched normal lung tissues. Strong correlation between mRNA levels of the particular genes was found, suggesting common patterns of their regulation. Only a desmoplakin encoding gene DSP was upregulated (p < 0.0001). In most cases, no association was identified between the expression of the genes and patients' clinicopathological characteristics. The only exceptions were more significant decreases in mRNA level of the PDPN (p = 0.002), FOX2 (p = 0.035) and NRP2 (p = 0.022) in smaller tumors and PROX1 (p = 0.02) and ITGA9 (p = 0.007) in squamous cell compared to adenocarcinoma tumors.

Conclusions

Our results seem to suggest a lack of the activation of the process of lymphangiogenesis in NSCLC.

Disclosure

All authors have declared no conflicts of interest.