218P - Truncated EpCAM is associated with a more aggressive phenotype and predicts poor overall survival in colorectal cancer

Date 28 September 2014
Event ESMO 2014
Session Poster Display session
Topics Colon Cancer
Rectal Cancer
Translational Research
Presenter Andreas Seeber
Citation Annals of Oncology (2014) 25 (suppl_4): iv58-iv84. 10.1093/annonc/mdu326
Authors A. Seeber1, G. Spizzo2, L. Terracciano3, A. Lugli4, N. Steiner1, G. Mazzoleni5, G. Gastl1, D. Fong2
  • 1Hematology And Oncology, University Hospital Innsbruck, 6020 - Innsbruck/AT
  • 2Onco-haematologic Day Hospital, Ospedale Franz Tappeiner, 39012 - Merano/IT
  • 3Pathology, University Hospital Basel, CH-4031 - Basel/CH
  • 4Pathology, Translational Research Unit, 3010 - Bern/CH
  • 5Pathology, Hospital of Bolzano, 39100 - Bolzano/IT

Abstract

Aim

Regulated intramembrane proteolysis (RIP) has been shown to be an important mechanism for oncogenic activation of EpCAM through nuclear translocation of the intracellular domain EpICD and consequently upregulation of c-myc. Recently, we identified two different membranous EpCAM variants namely EpCAMMF and EpCAMMT to be expressed in the majority of human epithelial carcinomas. The aim of our study was to evaluate the potential of these two candidate protein biomarkers for identification of aggressive colorectal cancer.

Methods

Using immunohistochemistry, we analyzed the expression of EpCAMMF and EpCAMMT variants in 632 patients with colorectal cancer and determined their correlations with other prognostic factors and clinical outcome.

Results

A statistically significant association was observed for EpCAMMT with advanced tumor stage (p < 0.001), histological grade (p < 0.001), vascular (p < 0.001) and marginal (p < 0.001) invasion using chi-square test. Kaplan-Meier survival analysis demonstrated reduced overall survival (p < 0.001) in patients showing the EpCAMMT phenotype when compared to patients with tumors expressing the EpCAMMF variant.

Conclusions

In conclusion, this study indicates for the first time that expression of EpCAMMT is associated with a more aggressive phenotype and predicts poor survival in patients with colorectal cancer.

Disclosure

All authors have declared no conflicts of interest.