1656P - Study of oxidative metabolism in tumor tissues of squamous cell lung cancer and adenocarcinoma

Date 28 September 2014
Event ESMO 2014
Session Poster Display session
Topics Lung and other Thoracic Tumours
Translational Research
Presenter Elena Shalashnaya
Citation Annals of Oncology (2014) 25 (suppl_4): iv564-iv573. 10.1093/annonc/mdu359
Authors E. Shalashnaya1, I. Goroshinskaya1, S. Chilingaryants2, E.I. Surikova1, P.S. Kachesova1, L.A. Nemashkalova1, A. Leonova1
  • 1Laboratory Of Research Of Pathogenesis Of Malignant Tumors, Rostov Research Oncological Institute, 344037 - Rostov-on-Don/RU
  • 2Department Of Oncosurgery, Medical Centre of Prof. Kruglov, 344037 - Rostov-on-Don/RU

Abstract

Aim

Comparative study of indices of free-radical oxidation and antioxidant system in tissue of squamous cell cancer and lung adenocarcinoma was conducted.

Methods

Activity of superoxide dismutase (SOD), catalase, glutathione peroxidase (GPx), glutathione reductase (GR), glutathione transferase (GST), content of reduced glutathione (GSH) and thiobarbituric acid reactive substances (expressed in terms of malondialdehyde) were studied by standard methods in tumor samples obtained during the surgery of 10 patients with squamous cell lung cancer and 12 patients with lung adenocarcinoma. Samples of healthy lung tissue obtained from 16 people without oncopathology (autopsy material) served as control.

Results

The analysis of the results showed that SOD activity exceeded the control by 32% in squamous cell cancer tissues only. Catalase activity in both types of tumor tissues did not differ from the control, while activity of glutathione-dependent enzymes was increased in both squamous cell cancer tissue and adenocarcinoma tissue: GPx – by 2.3 and 2.4 times; GST – by 52% and 2.3 times; GR – by 58% and 89% respectively. GSH content was significantly higher than the control – by 5.8 times in squamous cell cancer tissue and by 6.5 times in adenocarcinoma tissue. Activation of components of antioxidant protective system resulted in reduce of malondialdehyde content in squamous cell cancer tissue and adenocarcinoma tissue – by 1.8 times and 2.4 times respectively as compared to the level in the control.

Conclusions

Dysfunction of oxidative metabolism is revealed in non-small cell lung cancer tissue, being more expressed in adenocarcinoma, which may be one of the factors responsible for more aggressive biological behavior of lung adenocarcinoma - ability to metastasize early.

Disclosure

All authors have declared no conflicts of interest.