1638P - Steroid sulfatase promotes invasion and is a useful marker for predicting the progression of bladder cancer

Date 28 September 2014
Event ESMO 2014
Session Poster Display session
Topics Urothelial Cancers
Translational Research
Presenter Shimomura Eri
Citation Annals of Oncology (2014) 25 (suppl_4): iv564-iv573. 10.1093/annonc/mdu359
Authors S. Eri, M. Kato, M. Wei, S. Yamano, M. Fujioka, H. Wanibuchi
  • Department Of Pathology, Osaka City University graduate school of Medicine, 545-8585 - Osaka/JP

Abstract

Aim

Objectives-To assess the clinical and functional significance of steroid sulfatase (STS) in bladder cancer. STS is steroid sulfates activation enzyme and is considered one of the key enzymes in androgen signaling pathway. Androgen signal is recently suggested to be involved in the growth of bladder cancer. However, the role of STS in bladder cancer has not been elucidated.

Methods

STS expression was measured in 170 bladder cancer tissues by immunohistochemistry. The effect of STS knockdown on cell proliferation, migration and invasion capacity was evaluated using bladder cancer cell line (T24).

Results

The incidences of STS positive cancers were 21.3% and 41.2% in non-muscle invasive and muscle invasive bladder cancers, respectively (p = 0.0262). STS positive cancers showed shorter recurrence-free survival and cancer specific survival (CSS) (p = 0.0083, 0.0014, respectively). By multivariate analysis, STS expression level was identified as an independent prognostic factor for CSS (p = 0.043). Furthermore, in vitro knockdown of STS significantly reduced cell migration and invasion capacities of bladder cancer cells accompanied by up-regulation of E-cadherin and down-regulation of vimentin. However, the expression of androgen receptor (AR) was not correlated with that of STS, pathological stage or survival of patients with bladder cancer, suggesting that AR is not likely to play an important role in the progression of bladder cancer.

Conclusions

The present study demonstrates that STS promotes the invasion capability of bladder cancer and is a useful marker for predicting the progression of bladder cancers.

Disclosure

All authors have declared no conflicts of interest.