1652P - Influence of allogeneic mesenchymal stem cells on metastatic potential and tumor-infiltrating lymphocytes

Date 28 September 2014
Event ESMO 2014
Session Poster Display session
Topics Cancer Immunology and Immunotherapy
Translational Research
Presenter Tetiana Nikolaienko
Citation Annals of Oncology (2014) 25 (suppl_4): iv564-iv573. 10.1093/annonc/mdu359
Authors T.V. Nikolaienko1, L. Kladnytska2, L. Garmanchuk3, V.V. Nikulina3
  • 1Biochemistry, Taras Shevchenko National University of Kyiv, Educational and scientific centre “Institute of biology”, 03022 - Kyiv/UA
  • 2Biochemistry, 2Ukrainian education And Research Institute of Bioresourses Quality and Life Safety, National University of Life and Environmental Sciences of Ukraine, Kyiv/UA
  • 3Biochemistry, Taras Shevchenko National University of Kyiv, Educational and scientific centre “Institute of biology”, Kyiv/UA

Abstract

Aim

Today the use of stem cells in anti-cancer therapy is discussed extensively. The mesenchymal stem cells (MSCs) have the ability to form a cancer stem cell niche in which tumor cells can preserve the potential to proliferate and sustain the malignant process. The purpose of this study was to investigate the effect of allogeneic mesenchymal stem cell on biological characteristics of the primary tumor, lymphocyte infiltration degree of tumor in mice with transplanted Lewis lung carcinoma.

Methods

The investigation was carried out in C57Bl/6 male mice weighing 20-22g aged 2 to 3 months. Mice of the experimental group received the course of inoculation of MSCs in concentration 1,25x104 cells were administrated on 8th day after tumor cell inoculation. Metastatic volume in lung was assessed by determining the linear dimensions of metastases and their gradation in size (< 0,5mm; 1 mm; >1,5 mm). The lymphocyte infiltration degree of the tumor (cells/1 g tumor tissue) was estimated after their fractionation on gradient ficoll-verografin with density 1,077 g/ml by centrifugation 40 min at 1.5 thousand rpm.

Results

We found that the administration of MSCs to the animal with transplantable Lewis carcinoma increased the tumor weight by 41,7% vs control, and metastasis volume by 2,8 time vs control. Thess results show that MSCs increase tumor progression. Especially this effect is expressed by metastasis. Almost threefold enhancement of metastasis may be conditioned by the fact that MSCs enhance the angiogenic potential and create a niche for metastasis. It was found that the number of metastases more than 0.5 mm was increased almost twice after MSCs introduction compared with the control. This result shows an increase of agiogenesis in metastases under the influence of MSCs. This fact also is confirmed by enhancement of tumor infiltration by lymphocytes. Tumor-associated lymphocytes are also participants of angiogenesis, as a producers of VEGF. In determining the level of tumor infiltration by lymphocytes a significant increase (iof 1.3times) of the indicator in animals with MSCs was observed versus the relevant controls.

Conclusions

MSCs increase tumor progression and enhancement of tumor infiltration by lymphocytes. The presence of tumor-infiltrating lymphocytes has prognostic value that should be incorporated into the evaluation of the primary tumor.

Disclosure

All authors have declared no conflicts of interest.