1639P - Hormone level in uterine carcinoma tissue associated with different viral infections

Date 28 September 2014
Event ESMO 2014
Session Poster Display session
Topics Endometrial Cancer
Translational Research
Presenter Valeria Bandovkina
Citation Annals of Oncology (2014) 25 (suppl_4): iv564-iv573. 10.1093/annonc/mdu359
Authors V. Bandovkina1, T. Moiseenko2, E. Frantsiyants1, E. Komarova1, M. Vovkochina2
  • 1Laboratory Of Pathogenesis Of Malignant Tumors, Rostov Research Oncological Institute, 344037 - Rostov-on-Don/RU
  • 2Department Of Gynecology, Rostov Research Oncological Institute, 344037 - Rostov-on-Don/RU

Abstract

Aim

Unpredictability of tumor biological behavior may be determined by the involvement of different infectious agents in carcinogenesis. Malignant tumor's independent synthesis of hormones contributes to autonomy of growth and absence of central regulation. The question of the influence of infectious agents on hormonal status of malignant uterine tumors still remains open.

Methods

Levels of estrone (Е1), estradiol (Е2), testosterone (T), prolactin (PRL) and progesterone (Р4) were assessed in 41 tumor samples obtained during operative therapy of patients with endometrial cancer T1-2N0M0 in 10% homogenates with enzyme-linked immunosorbent assay (ELISA) with the use of standard test systems (XEMA). Mean age was 53.4±3.2 years, histological structure - endometrioid adenocarcinoma. IgG antibodies against herpes simplex virus (HSV), cytomegalovirus (CMV) and chlamydias were detected in tumor tissue with ELISA. The polymerase chain reaction method was used for the detection of CMV, HSV and chlamydia infection in ovarian tissue. Adenocarcinoma without IgG against infectious agents served as a control.

Results

IgG against HSV antigens were found in 26.8% of the cases; IgG against CMV+HSV antigens+clamydias – in 17.1%; IgG against CMV antigens and IgG against CMV+HSV antigens – in 12.2%; 31.7% of the tissues did not have IgG against the infections. It was found that the infections alone or in combinations resulted in the increase of Е1 level by 1.3-1.7 times, Е2 by 1.6-1.9 times, PRL by 3 times while T concentration decreased by 1.4-3.4 times in comparison with adenocarcinomas without infectious agents. Р4 concentration in infected tumors did not differ significantly from the indices in uninfected ones remaining reduced in comparison with intact endometrium tissue.

Conclusions

Infections in tumor tissue, alone or in combinations, cause additional potential for changes and precarious states that can result in drug resistance, resistance to treatment, and reduction of differentiation, that is an increase of malignancy.

Disclosure

All authors have declared no conflicts of interest.