13P - Expression of IRAK-1 and EZH-2 in imprints of NSCLC and preneoplastic lesions

Date 17 April 2015
Event ELCC 2015
Session Poster lunch
Topics Non-Small-Cell Lung Cancer, Metastatic
Translational Research
Presenter Kyriakos Hainis
Citation Annals of Oncology (2015) 26 (suppl_1): 1-5. 10.1093/annonc/mdv043
Authors K. Hainis1, A. Athanassiadou2, E. Haini1, A. Tsipis2, M. Gonidi3, P. Athanassiadou2
  • 1Pulmonary, Corfu General Hospital, 49100 - Corfu/GR
  • 21st Pathology Department And Cytology Unit, National and Kapodistrian University of Athens, Athens/GR
  • 3Cytology, Corfu General Hospital, Corfu/GR

Abstract

Aim/Background

Interleukin – 1 receptor associated kinase 1 (IRAK-1) is increasingly being recognized as an important mediator in cancer initiation and progression. Enhancer of Zeste Homolog 2 (EZH 2) promotes carcinogenesis by epigenetically silencing tumor suppressor genes. We studied IRAK-1 and EZH-2 expression in non-small cell lung carcinoma (NSCLC) and corresponding preneoplastic lesions.

Methods

Imprint smears from 102 NSCLC (adenocarcinomas and squamous cell carcinomas) and adjacent atypical squamous metaplasia (n = 20) and normal bronchial epithelium (n = 20) were studied for the immunocytochemical expression of IRAK-1 and EZH2. The results were correlated with patients' clinicopathologic features. Furthermore we investigated the correlation between IRAK-1and EZH-2 expression in tumour imprint specimens.

Results

NSCLC tumors demonstrated significantly cytoplasmic and lower nuclear IRAK-1 expression and higher nuclear expression for EZH-2 than normal epithelium. Atypical squamous metaplasia had significantly higher cytoplasmic IRAK-1 and nuclear EZH-2 expression. In tumor specimens; significant positive correlation was detected between IRAK-1 expression and EZH2 (p < 0,0001). The correlation between the expression IRAK-1 and EZH-2 and patients clinicopathologic features varied according to histological type of the tumor and the grade.

Conclusions

IRAK-1 and EZH-2 are expressed in high percentages in NSCLC imprint smears and their expression in specimens with atypical squamous metaplasia is an early phenomenon in the sequential development of lung cancer.

Disclosure

All authors have declared no conflicts of interest.