69P - Diagnostic and prognostic role of stromal CD 10 and Ki 67 in benign and malignant phylloides tumor of breast: A pilot study

Date 07 May 2015
Event IMPAKT 2015
Session Welcome reception and Poster Walk
Topics Breast Cancer
Translational Research
Presenter Surender Kumar
Citation Annals of Oncology (2015) 26 (suppl_3): 15-24. 10.1093/annonc/mdv117
Authors S. Kumar1, D. Singh1, S.K. Gupta1, V.K. Singh2
  • 1Surgery, CSM Medical University (KG's Medical University) Gandhi Memorial & Associated Hospital, 226003 - Lucknow/IN
  • 2Social And Preventive Medicine, CSM Medical University (KG's Medical University) Gandhi Memorial & Associated Hospital, 226003 - Lucknow/IN

Abstract

Body

Background: Phylloides tumors (PT) are rare fibroepithelial tumor of breast, closely resemble to breast fibroadenoma, ranging from benign to malignant tumor. PT has potential to recur and metastasize due to its stromal characteristics. Differentiation between benign and malignant tumor is not possible with histopathology alone, additional study of stromal CD 10 and Ki 67 antigen expression in PT may be useful to differentiate benign from malignant tumor.

Method: Immunohistochemical study using specific monoclonal antibody to label stromal CD 10 and Ki 67 antigen were done in tissue sample of 35 patients.

Result: Out of total 35 patients histopathologically 18 were benign, 9 borderline and 8 were malignant PT. On immunohistochemistry 16 patients had CD 10 expression labeled as CD 10 positive (7 malignant, 7 borderline and 2 benign), and 19 were CD 10 negative (1 malignant, 2 borderline and 16 benign) (p < 0.000). Overall sensitivity, specificity, PPV and NPV to diagnose malignant PT was 82.35%, 88.88%, 77.77% and 94.11% respectively. 23 patients had Ki 67 labeling index between 1 and 8 (18 benign, 5 borderline) and 12 patients had index > 8 (8 malignant, 4 borderline) i.e. no one malignant PT had Ki 67 labeling index < 8 (p <0.000). Sensitivity, specificity, PPV and NPV for diagnosing malignant PT were 100%.

Conclusion: Stromal CD 10 and Ki67 expression is higher in malignant PT. Ki 67 labeling index >8 are strongly suggestive of malignant PT and needs to appropriate treatment and close follow-up even in histopathologically benign tumor.

Disclosure: All authors have declared no conflicts of interest.