1603P - Adipose-derived stem cells with the control release VEGF polylactic acid fiber catheters gelatin scaffold for tissue engineering

Date 28 September 2014
Event ESMO 2014
Session Poster Display session
Topics Translational Research
Presenter Longlong Ding
Citation Annals of Oncology (2014) 25 (suppl_4): iv546-iv563. 10.1093/annonc/mdu358
Authors L. Ding1, F. Wu2, Y. Lu1, L. Li2, Y. Jia1, T. Jin2, K. Wu1
  • 1General Surgery, XinHua Hospital, Shanghai Jiaotong University School of Medicine, 200092 - Shanghai/CN
  • 2College Of Pharmacy, Shanghai Jiaotong University School, Shanghai/CN

Abstract

Aim

The major obstacles of autologous fat grafting after surgery for breast cancer were low graft survival and high resorption rate. The purpose of this study was to explore adipose-derived stem cells with a new scaffold could control release vascular endothelial growth factor (VEGF) to promote transplant survival and neovascularization.

Methods

Polylactic acid fiber catheters loaded with VEGF were included in a gelatin scaffold produced by freeze crosslinking. The release dose and persistence of VEGF from the tridimensional structures were determined using the enzyme-linked immunosorbent assay (ELISA). Human adipose-derived stem cells (hASCs) were extracted and expanded ex vivo, seeded to the scaffold and cultured to test the biocompatibility using the WST-1 assay. Three days after co-cultured with hASCs, the scaffolds were transplanted to the back of nude mice, the control groups were only inject hASCs, transplanted control scaffold with hASCs. Three months later, transplants volume and histology were evaluated and neovascularization was determined by the quantity of capillary and the positive of endothelial cell marker CD31 in immunohistochemistry.

Results

The tridimensional structures could release VEGF stable and durable, which had no significant cytotoxicity on hASCs. The transplants volume and the capillary quantity of hASCs with the control release VEGF polylactic acid fiber catheters gelatin scaffold group was significantly larger than other two groups. Immunohistochemistry analysis revealed the transplants were rich in CD31-positive cells.

Conclusions

Adipose-derived stem cells together with the control release VEGF polylactic acid fiber catheters gelatin scaffold could significantly promoted vasculogenesis and reduced absorption and necrosis after transplant.

Disclosure

All authors have declared no conflicts of interest.