53P - A novel potential prognostic marker SIRT1 on tumor invasion and metastasis, and tumor recurrence in triple negative breast cancer

Date 07 May 2015
Event IMPAKT 2015
Session Welcome reception and Poster Walk
Topics Breast Cancer, Metastatic
Translational Research
Presenter HanSuk Ryu
Citation Annals of Oncology (2015) 26 (suppl_3): 15-24. 10.1093/annonc/mdv117
Authors H. Ryu1, I.A. Park2, H. Kim1, Y.Y. Jung3
  • 1Pathology, Seoul National University Hospital, 110-744 - Seoul/KR
  • 2Pathology, Seoul National University Hospital, Seoul/KR
  • 3Pathology, Samsung Medical Center Sungkyunkwan University School of Medicine, Seoul/KR

Abstract

Body

Background: Silent mating type information regulation 2 homolog 1 (SIRT1) is a histone deacetylase that regulates a variety of cellular and physiological events. But it is not well recognized how SIRT1 act on breast cancer. In this study, we investigated the prognostic role of SIRT1 expression on tumor invasion and lymph node metastasis, and disease related recurrence survival in triple negative breast cancer (TNBC).

Methods: 344 patients who received surgical resection of TNBC from January 2003 to December 2006 at Seoul National University were enrolled in this study. Clinicopathologic information was obtained by reviewing electronic medical records. Tissue microarray was used for SIRT1 immunohistochemistry. To study cancer cell invasion ability, western blot and invasion assay was carried out with Human TNBC cell line (MDA-MB-231), following SIRT1-siRNA transfection.

Results: The expression of SIRT1 significantly correlated with lymph node metastasis (P = 0.008). In multivariate analysis, SIRT1 (P = 0.011), T stage (P = 0.014) and lymphatic invasion (P < 0.001) was revealed independent prognostic factors. On receiver operating characteristics curves analysis, the value of areas under the curves was 0.69. SIRT1 expression correlated with shorter disease free survival (P = 0.003), but it did not show statistical significance on overall survival. On invasion assay, SIRT1-silenced TNBC cell line showed significantly reduced invasion ability.

Discussion: In TNBC, SIRT1 may have an important role on invasion and tumor progression, and could be used as a prognostic indicator as well as a potential chemotherapeutic target.

Disclosure: All authors have declared no conflicts of interest.