P-012 - Significance and relationship between the expression of Nodal-1 and YAP1 with Gastric Carcinogenesis and Metastasis

Date 04 July 2015
Event WorldGI 2015
Session Posters
Topics Gastric Cancer
Pathology/Molecular Biology
Translational Research
Presenter P. Li
Citation Annals of Oncology (2015) 26 (suppl_4): 1-100. 10.1093/annonc/mdv233
Authors P. Li, Y. Xin, D. Sun
  • the First Hospital of China Medical University, Shenyang/CN

Abstract

Introduction

Nodal-1 is a member of transforming growth factorß(TGF-ß) superfamily, which regulates the growth and differentiation of embryonic stem cells plasticity and aggressive of malignant tumor cells. Nodal expression has been found in a variety of tumor cells lines, such as metastatic melanoma, breast cancer, metastatic testicular cancer, and glioma. The upregulated expression of Nodal in a variety of malignant tumors indicates its close relation to tumor development. YAP1 is a transcriptional coactivator in Hippo pathway, which is involved in tumor development. The aim of this study is to detect the expression of Nodal-1 protein and YAP1 in normal gastric mucosa and gastric carcinoma (GC), and to analyse the differences of their expressions, and to explore the relations to gastric carcinogenesis and metastasis.

Methods

The expression of Nodal-1 and YAP1 at mRNA and protein level were detected by real time reverse transcription-polymerase chain reaction (qRT-PCR), and western blot (WB). Immunohistochemical method was used to detect the expression of Nodal-1 protein in 223 gastric carcinomas and normal gastric mucosa.

Results

The results of immunohistochemistry show that the positive rates of Nodal-1 and YAP1 expressions were significantly higher in gastric carcinoma than in normal gastric mucosa, respectively. The positive rate of Nodal-1 In GC with lymph node metastasis was significantly higher than those without metastasis, P < 0.05. The upregulated Nodal-1 expression in GC was also demonstrated in our western blot analysis. In 40 cases of gastric carcinoma, the expression of Nodal-1 and YAP1 protein was significantly positively correlated. The results of western blot and qRT-PCR showed that the expression level of Nodal-1 and YAP1 were higher in gastric cancer than in normal gastric mucosa at both mRNA and protein levels (P < 0.05).

Conclusion

The positive rates of Nodal-1 and YAP1 expression were significantly higher in gastric cancer than in normal gastric mucosa, indicating that they may play important roles as tumorigenic factors and early gastric tumorigenic molecules during gastric carcinogenesis. Our results showed a positive correlation between Nodal-1 and YAP1 expression and lymph node metastasis, indicating that they may participate in the development and lymph node metastasis of GC. Nodal-1 and YAP1 can act as a prognostic indicator for GC patients, but the relevant molecular mechanism requires further investigation.